Full Description
This volume presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.
Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research.
Contents
The Evolution of Immunocytochemistry in the Dissection of Neural Complexity.-n Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System.- Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris.- Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish Brain.- Immunocytochemical Phenotype of Differentiating Neurons.-Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus.- Identification of Proliferating and Migrating Cells by BrdU and Other Thymidine Analogues. Benefits and Limitations.- Flow Cytometry-Based Quantification of Neurogenesis in the Central Nervous System.- In Vivo and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis and Autophagy.- Immunocytochemical Detection of Intraneuronal Aβ Peptides in Mouse Models of Alzheimer's Disease.- Purification and Characterization of Mouse Olfactory Ensheathing Cells and their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS).- Immunocytochemistry of Microglial Cells.- Immunocytochemical Assessment of Blood-Brain Barrier Structure, Function, and Damage.- Quantification of Immunocytochemical Colocalization in Neurons.- Immunogold Electron Microscopic Quantification of Small Molecular Compounds and Proteins at Synapses and Other Neural Profiles.- Combined Immunocytochemistry and Tracing of Neural Connections.- Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing.- Transfection Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic Slices.- Combined Immunochemistry and Live-Imaging of Fluorescent Protein Expressing Neurons in Mouse Brain.- Array Tomography: A Novel High-Resolution Immunofluorescence Technique.- Single Molecule Localization Microscopy to Study Neuronal Microtubule Organization.-Studying Membrane Dynamics of Proteins Using Quantum Dots.- Optical Protein Patterning.
