Full Description
This volume is a collection of
research methods, techniques, and approaches to investigate the molecular
biology of West Nile Virus (WNV). Chapters in the book cover many facets of
WNV, such as propagation and titration of WNV in vero cells, examination of WNV
neuroinvasion and neuropathogenesis in the central nervous system of a murine
model, field surveillance methods for WNV, and in vitro and in vivo
blood-brain barrier models to study WNV pathogenesis. A brief introduction,
along with a discussion on WNV laboratory safety are also included. Written in
the highly successful Methods in Molecular Biology series format,
chapters includes introduction to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, West Nile Virus will interest virologists,
molecular biologists, microbiologists, and other scientists working with WNV.
This book is a comprehensive guide for those conducting research in the field
of WNV biology, including life cycles, pathogenesis, impact of infection, and
possible treatment/prevention development.
Contents
A Brief Review
of West Nile Virus Biology. Overview
of West Nile Virus Transmission and Epidemiology.- Propagation
and Titration of West Nile Virus on Vero Cells.- Generating West Nile Virus from an
Infectious Clone.- Functional
Analysis of West Nile Virus Proteins in Human Cells.- Lessons
from the Murine Models of West Nile Virus Infection.- Exploration
of West Nile Virus Infection in Mouse Models.- Examination
of West Nile Virus Neuroinvasion and Neuropathogenesis in the Central Nervous System
of a Murine Model.- In Vitro and In Vivo Blood-Brain Barrier Models to Study West Nile Virus Pathogenesis.- Methodology for Identifying Host Factors Involved
in West Nile Virus Infection.- ELISA and
Neutralization Methods to Measure Anti-West Nile Virus Antibody Responses.- Safe Handling
of West Nile Virus in the Insectary.- Techniques for Experimental Infection of Mosquitoes
with West Nile Virus.- Field Surveillance Methods for West Nile Virus.- Detection
Protocols for West Nile Virus in Mosquitoes, Birds, and Non-Human Mammals.- Field Methods and Sample Collection Techniques
for the Surveillance of West Nile Virus in Avian Hosts.- Statistical
Tools for the Interpretation of Enzootic West Nile Virus Transmission Dynamics.- Role of Immune
Aging in Susceptibility to West Nile Virus.- An Overview
of Current Approaches Toward the Treatment and Prevention of West Nile Virus Infection.
