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Full Description
Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.
Contents
An Introduction to Plant-Cell Culture.- Cell Culture and Plant Regeneration.- Callus Initiation, Maintenance, and Shoot Induction in Rice.- Callus Initiation, Maintenance, and Shoot Induction in Potato.- Somatic Embryogenesis in Barley Suspension Cultures.- Somatic Embryogenesis in Picea Suspension Cultures.- Direct, Cyclic Somatic Embryogenesis of Cassava for Mass Production Purposes.- Immature Inflorescence Culture of Cereals.- Cryopreservation of Rice Tissue Cultures.- Noncryogenic, Long-Term Germplasm Storage.- Plant Propagation In Vitro.- Micropropagation of Strawberry via Axillary Shoot Proliferation.- Meristem-Tip Culture for Propagation and Virus Elimination.- Clonal Propagation of Orchids.- In Vitro Propagation of Succulent Plants.- Micropropagation of Flower Bulbs.- Clonal Propagation of Woody Species.- Spore-Derived Axenic Cultures of Ferns as a Method of Propagation.- Applications for Plant Protoplasts.- Protoplast Isolation, Culture, and Plant Regeneration from Passiflora.- Isolation, Culture, and Plant Regeneration of Suspension-Derived Protoplasts of Lolium.- Protoplast Fusion for Symmetric Somatic Hybrid Production in Brassicaceae.- Production of Cybrids in Rapeseed (Brassica napus).- Microprotoplast-Mediated Chromosome Transfer (MMCT) for the Direct Production of Monosomic Addition Lines.- Guard Cell Protoplasts.- In Vitro Fertilization with Isolated Single Gametes.- Protocols for Genomic Manipulation.- Protocols for Anther and Microspore Culture of Barley.- Microspore Embryogenesis and In Vitro Pollen Maturation in Tobacco.- Embryo Rescue Following Wide Crosses.- Mutagenesis and the Selection of Resistant Mutants.- The Generation of Plastid Mutants In Vitro.- Protocols for the Introduction of Specific Genes.- Agrobacterium-Mediated Transformation of Petunia Leaf Disks.- Transformation of Rice via PEG-Mediated DNA Uptake into Protoplasts.- Transformation of Wheat via Particle Bombardment.- Plant Transformation via Protoplast Electroporation.- Transformation of Maize via Tissue Electroporation.- Transformation of Maize Using Silicon Carbide Whiskers.- Suspension Culture Initiation and the Accumulation of Metabolites.- Directing Anthraquinone Accumulation via Manipulation of Morinda Suspension Cultures.- Alkaloid Accumulation in Catharanthus roseus Suspension Cultures.- Betalains.
