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Full Description
This volume is based on an international symposium held during September 9-12, 1986 in Bocca di Magra, Italy. The intent of the organizers was to bring together expert practitioners of fluorescence spectroscopy, particularly as applied to biological systems, to assess recent developments in the field and discuss future directions. At the same time the meeting was intended to honor the singular and outstanding scientific career of Gregorio Weber on the occasion of his seventieth birthday. Gregorio Weber is truly the pioneer in the application of fluores cence methods to biochemistry and biophysics. A complete list of his scientific contributions to fluorescence and to protein biochemistry is beyond the scope of this preface. Suffice it to say that since his initial landmark articles on fluorescence, published in the late 1940's and early 1950's, Gregorio Weber has continued to make seminal contribu tions to both the theory and practice of fluorescence and, contrary to many who might be tempted to rest on their laurels, he shows no signs of slackening his pace. In addition to his more obvious tangible contributions to the scientific field, Gregorio Weber has made equally valuable contributions of another type. Specifically, he has had the most profound impact, both profeSSionally and personally, on generations of young scientists.
Contents
Luminescence: General Concepts and Applications to the Study of Solids.- Protein Dynamics: Fluorescence Lifetime Distributions.- Enhancement of Time-Resolved Fluorescence Spectroscopy by Overdetermination.- Fluorescence Investigations on the Elongation Factor Tu System.- Effects on Intrinsic Fluorescence Induced by Active Site Modifications of Rhodanese.- Lifetime Measurements of Color Centers by Fluorometry with Synchrotron Radiation.- Slab Gel Electrophoresis Performed at High Hydrostatic Pressure: A New Approach for the Study of Oligomeric Proteins.- Lipid Transfer Reactions: Fluorescence Studies.- Thoughts and Hints on Possible Violation of Parity in the Biological Matter.- Digitized Fluorescence Microscopy in Cell Biology Applications.- Membrane Fluidity and Diffusive Transport.- Conformational Flexibility of the Polypeptide Hormone Bombesin: Time Resolved and Static Fluorescence Studies.- Usefulness of the Linkage Concept to Understanding the Regulation of Rat Liver Phosphofructokinase.- Fluorescence Studies of the Calcium-Dependent Functions of Calmodulin.- Definition of Monoclonal Antibody-Fluorescent Ligand Interactions.- Intramolecular Excitation Energy Transfer in Bichromophoric Molecules — Fundamental Aspects and Applications.- Resolution at 2 GHz of Lifetimes and Correlation Times of Highly Purified Solutions of Human Hemoglobin.- Time-Resolved Fluorescence Studies on Flavins.- Final Words at Bocca di Magra.- Abstracts from Poster Contributions.- Participants.- Author Index.
