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Full Description
This concise, truncated version of Nagy, Varghese and Agarwal's Practical Manual of In Vitro Fertilization is comprised of select clinical chapters for a portable, affordable and up-to-date resource. Clinical Embryology covers a variety of topics, including:
- Sperm processing methods
- Embryo culture methods
- Insemination procedures
- Embryo evaluation
- Genetic testing of embryos
- Cryopreservation
- Embryo transfer
Also included are two new chapters on predicting embryo developmental potential and viability using automated time-lapse analysis (Eeva) and predicting embryo implantation potential using Embryoscope.™
Practical for both clinicians and researchers alike, Clinical Embryology brings together all of the need-to-know information about these important topics in reproductive medicine.
Contents
Journey of Human Gametes In Vitro: 1978 to 2010.- Part I: Sperm Processing Methods.- Sperm Processing for IVF.- PESA/TESA/TESE Sperm Processing.- Processing Sperm Samples in HIV-Positive Patients.- Part II: Embryo Culture Methods.- Culture Media in IVF: Decisions for the Laboratory.- Short Culture: Day 1/Day 2/Day 3 Embryo Culture.- Extended Culture in IVF.- In Vitro Maturation of Human Oocytes.- In Vivo Embryo Culture Device.- Microfluidics for Gamete Manipulation and Embryo Culture.- Part III: Insemination Procedures.- Intrauterine Insemination.- Conventional IVF Insemination.- Oocyte Denuding.- Intracytoplasmic Sperm Injection.- Part IV: Embryo Evaluation and Grading.- Pronuclear Scoring in Human In Vitro Fertilization.- Cumulative Morphological Assessment of Embryo Quality.- Metablomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability.- Oxygen Consumption as an Indicator of Oocyte and Embryo Viability.- Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection.- Amino Acid Turnover as a Biomarker of Embryo Viability.- Real-Time Embryo Monitoring Devices for Embryo Selection.- Predicting Embryo Developmental Potential and Viability Using Automated Time-Lapse Analysis (Eeva Test).- Predicting Embryo Implantation Potential Using Video Monitoring by the Embryoscope™ Time-Lapse System.- Part V: Genetic Testing of Embryos.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosomal Abnormalities and Gene Defects.- Part VI: Cryopreservation.- Sperm Cryopreservation.- Slow Freezing of Oocytes.- Vitrification: Research in Animal Models.- Oocyte Vitrification.- Slow Freezing of Embryos.- Vitrification of Embryos.- Directional Freezing of Reproduction Cells, Tissues, and Organs.- Ovarian Tissue Cryopreservation.- Part VII: Embryo Transfer.- Assessment of Uterine Receptivity.- Single Embryo Transfer.- Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-BasedEvaluation.
