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Full Description
The book provides a comprehensive guide that covers the fundamental principles and methodologies of essential bioanalytical techniques.
Bioanalytical Techniques: Principles and Applications is a comprehensive and authoritative book that explores the principles, methodologies, and applications of bioanalytical techniques in the field of life sciences. The book covers a wide range of analytical techniques used for the characterization, quantification, and analysis of biological samples, including proteins, nucleic acids, metabolites, and biomarkers. Using a multidisciplinary approach by integrating concepts from biochemistry, molecular biology, analytical chemistry, and biotechnology, this book provides a solid foundation in the fundamental principles underlying various bioanalytical techniques, such as spectroscopy, chromatography, electrophoresis, immunoassays, mass spectrometry, and biosensors. Each technique is explained in detail, including its working principles, instrumentation, data analysis, and practical considerations. The book incorporates case studies, examples, and practical tips to illustrate how these techniques are used to solve biological problems and address research questions. It also discusses emerging trends and technologies in bioanalytical techniques, such as microfluidics, nanotechnology, and omics approaches.
Readers will find the book:
Offers comprehensive coverage of bioanalytical techniques, encompassing a wide range of methodologies, instruments, and applications through real-world case studies;
Adopts a multidisciplinary approach, integrating concepts from biochemistry, molecular biology, analytical chemistry, and biotechnology;
Explores emerging trends and technologies in bioanalytical techniques, such as microfluidics, nanotechnology, omics approaches, and bioinformatics;
Includes practical guidance, troubleshooting tips, and common challenges in bioanalysis, equipping readers with valuable insights and strategies for successful experimentation and data interpretation;
Features contributions from renowned experts and leaders in the field, ensuring the content is authoritative, up-to-date, and reflects the latest advancements in bioanalytical techniques.
Audience
Biochemists, biologists, chemists, and medical and pharmaceutical professionals interested in biomolecules, enzymology, and biochemical pathways.
Contents
Preface xxix
1 Point-of-Care Diagnostics for Rapid Disease Detection 1
Nabil H. Bhuiyan and M. Khalid Hossain
2 Principles of Molecular Biology for Bioanalysis 39
Syed Ali Raza Naqvi, Syeda Marab Saleem, Tania Jabbar, Atta Ul Haq, Muhammad Asif, Sadaf Ul Hassan, Muhammad Rehan Hasan Shah Gilani, Naseem Abbas and Ali Abbas
3 UV-Visible Spectroscopy for Quantitative Analysis of Biomolecules 79
Syeda Marab Saleem, Syed Ali Raza Naqvi, Muhammad Ramzan Saeed Ashraf Janjua, Tauqir A. Sherazi, Muhammad Saeed, Sadaf Ul Hassan, Muhammad Imran, Muhammad Asif, Mamoon Ur Rasheed and Tania Jabbar
4 Emerging Trends and Future Perspectives in Bioanalysis 119
Jayapal Reddy Gangadi, Kalpana Swain and Satyanarayan Pattnaik
5 Introduction to Biosensors for Point]of-Care Testing: IoT-Based Vitamin D3 Biosensor 137
Asif Hussain Shaik and Chinna Babu Jyothi
6 Fluorescence Spectroscopy for Protein-Protein Interactions 149
Masoud Shayegan and Hossein Ahmadzadeh
7 Flow Cytometry for Cell Analysis and Sorting 173
Maryam Davardoostmanesh and Hossein Ahmadzadeh
8 Application in Drug Discovery and Development for Lead Identification 189
Sareena Ehsan, Rida Mumtaz, Abdul Rehman, Maryam, Habibullah Nadeem, Ammara Riaz, Jallat Khan, Ebrahim Alinia-Ahandani and Zeliha Selamoglu
9 Immunoblotting Techniques for Protein Identification and Quantification and Its Application 203
Megha Sikder, Somen Debnath and Biswanath Bhunia
10 Gel Electrophoresis for DNA Fragment Analysis 221
Aneela Nawaz, Shahzar Khan, Momina Aamir, Sidra Zaheer, Sabeena Zafar, Nauman Ahmed Khalid and Samiullah Khan
11 Omics Approaches in Bioanalysis for Systems Biology Studies 239
Rana Hooshang, Hoda Nouri and Hamid Moghimi
12 Introduction to Mass Spectrometry for Metabolite Profiling 283
Jackie E. Wood and Brendon D. Gill
13 Infections, Symptoms, and Clinical Diagnostic Techniques for Dengue: A Case Study of a Neglected Tropical Disease 341
Shreeganesh Subraya Hegde and Badekai Ramachandra Bhat
14 Microfluidic-Based Bioassays for High-Throughput Analysis 355
Muthui Martin Mwaurah, J. Mathiyarasu and A.M. Vinu Mohan
15 Circular Dichroism Spectroscopy for Secondary Structure Analysis of Proteins 383
Masooma Siddiqui, Maroof Ali, Mehak Sagheer and Mohd Imran Ahamed
16 Optical Biosensors for Detection of Analytes in Biological Samples 427
Uzma, Masooma Siddiqui and Mehtab Parveen
17 Tandem Mass Spectrometry for Peptide Sequencing 461
Satyendra Mishra and Neha V. Rathod
18 Unraveling Complexity: Cutting-Edge Ion Chromatography Methods for the Analysis of Inorganic Ions in Biological Samples 489
Muhammad Hafiznur Yunus, Nor Ain Shahera Khairi, Azren Aida Asmawi, Fatin Nabilah Mohd Faudzi, Ahmad Farabi Mohamad Saman, Nor Azah Yusof and Siti Fatimah Nur Abdul Aziz
19 Sample Preparation and Handling for Reliable Results 551
Virender, Rohit, Ashwani Kumar, Rakesh Kumar Gupta and Brij Mohan
20 Data Analysis in Bioanalysis for Interpretation of Experimental Data 575
Jaymin Parikh, Keyur Bhatt, Krunal Modi, Jasbir Sangwan, Alexander A. Solovev and Brij Mohan
21 Protein Identification Using Matrix-Assisted Laser Desorption/ Ionization Mass Spectrometry 605
Vipul D. Prajapati and Princy Shrivastav
22 Applications of Electrothermal Atomic Absorption Spectrometry (ET-AAS) Toward Various Metal Analyses in Diverse Biological and Environmental Matrices 629
Aruna Jyothi Kora
23 Thin-Layer Chromatography for Natural Product Analysis 645
Mahreen Imam, Syed Ali Raza Naqvi, Majid Muneer, Atta Ul Haq, Sadaf Ul Hassan, Muhammad Rehan Hasan Shah Gilani, Naseem Abbas and Syeda Marab Saleem
Index 667
