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Full Description
This laboratory manual for allied health or general microbiology has been written with the student in mind. The authors have used their years of teaching microbiology and microbiology laboratory at all levels to identify and relate the fundamental concepts that are important to the understanding of the science and students' success in their future field. They have included case studies to exemplify the relevance of the science and extensive visual imagery to help students understand and learn the content. Most importantly, the authors hope this manual will help students experience the thrill of bench science and share some of the enthusiasm they have for microbiology, a field of science that is dynamic, exciting and touches every aspect of your life.
Contents
Laboratory OverviewMaterialsBasic TechniquesEx 1 Use of the MicroscopeEx 2 Aseptic TechniquesEx 3 Isolation Streak PlateSurvey of the Microbial WorldEx 4 Microbial Phototrophs: Algae and CyanobacteriaEx 5 ProtozoansEx 6 The FungiEx 7 Motility: Hanging Drop MethodStaining TechniquesEx 8 Introduction to StainingEx 9 Negative StainEx 10 Capsule StainEx 11 Gram StainEx 12 Acid-Fast Staining (Ziehl-Neelsen and Kinyoun Methods)Ex 13 Endospore Stain (Schaeffer-Fulton Method)Media: Enriched, Differential, and SelectiveEx 14 Blood AgarEx 15 Mannitol Salt AgarEx 16 Eosin Methylene Blue AgarEx 17 MacConkey Agar Ex 18 Evaluation of MediaGrowthEx 19 Standard Plate CountEx 20 Osmotic GrowthEx 21 AerotoleranceEx 22 Catalase Ex 23 OxidaseControl of Microbial GrowthEx 24 Killing by Ultraviolet LightEx 25 Antimicrobial Susceptibility Test (Kirby-Bauer Method)Ex 26 Hand WashingDiagnostic GeneticsEx 27 DNA Fingerprinting Applied GeneticsEx 28 Transformation Microbes & Disease Ex 29 Parasitic Protozoans Ex 30 Parasitic WormsEx 31 Vectors of DiseaseDiagnostic ImmunologyEx 32 Gel Immunoprecipitation (Immunodiffusion)Ex 33 Passive (Indirect) Agglutination Identifying MicrobesEx 34 EnteroPluri-TestEnvironmental MicrobiologyEx 35 Soil MicrobiologyHydrolytic EnzymesEx 36 Dnase Test Ex 37 Gelatin HydrolysisEx 38 Starch HydrolysisEx 39 Casien HydrolysisBiochemical TestingEx 40 Phenol Red BrothEx 41 Triple Sugar Iron AgarEx 42 Litmus MilkEx 43 IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate)Ex 44 Urease TestEx 45 Nitrate Reduction Putting It All TogetherEx 46 Known Bacteria for Biochemical TestingEx 47 Identification of Unknown BacteriaCreditsIndexNER(01): WOW
