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Full Description
Lipid Second Messengers provides detailed methodology for analysis of various lipid signaling pathways. Authoritative contributors explain the factors that regulate lipid second messenger production by agonist-activated enzymes and examine their products. Topics discussed include procedures used to measure lipid-derived mediators such as lysophospholipids, arachidonic acid, eicosanoids, anandamide, and ceramides, and the enzymes responsible for generating these messengers, such as phospholipases, prostaglandin endoperoxide synthases, and sphingomyelinase.
Contents
Preface -- Chapter-1 Phospholipase D /Louis Freysz and JulianN. Kanfer -- Chapter-2 Analyzing the Sphingomyelin Cycle: Protocols for Measuring Sphingomyelinase, Sphingomyelin, and Ceramide /Heather Hayter and Yusuf A. Hannun -- Chapter-3 Expression of the Human Prostaglandin Endoperoxide H Synthases: Measurement of Cyclooxygenase Activity and Inhibition by Nonsteroidal Anti-Inflammatory Drugs /David DeWitt -- Chapter-4 Phospholipid Growth Factors: Identification and Mechanism of Action /Gabor J. 1igyi, Karoly Liliom, David J. Fischer, and Zhong Guo -- Chapter-5 Leukotriene and Lipoxin Biosynthesis /Bruce D. Levy, Karsten Gronert, Clary Clish, and Charles N. Serhan -- Chapter-6 Purification and High-Resolution Analysis of Anandamide and Other Fatty Acylethanolamides /Andrea Giuffrida and Daniele Piomelli -- Index.



