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Full Description
A valuable resource for introducing flow cytometry, providing a foundation for understanding its importance in various fields
The newly updated and revised third Edition of Flow Cytometry: First Principles, 3rd Edition covers flow cytometric theory and the fundamentals of instrumentation and data analysis using examples from a wide range of scientific and clinical applications. Readers will find and learn about both standard and advanced flow cytometric instrumentation, methods for analyzing flow data, and applications of the technique ranging from immunology, clinical diagnoses, DNA analysis, and cell sorting, as well as novel applications such as microbiology, aquatic biology, and molecular biology. Each chapter starts with basic knowledge and gradually expands on the subject matter, enabling readers to assess the ways that flow cytometry has been used in their respective fields and the ways that it might be applied in the future. A selected number of annotated references are given, aiming to point readers into relevant literature with an extensive glossary at the end.
Written by two experts and highly illustrated, Flow Cytometry: First Principles, Third Edition includes information on:
How flow cytometry is used to analyze cells
How a flow cytometer can be used to separate different types of cells from each other
Fluidics, electronics, and optics (including lasers, fluorochromes, mirrors, and filters)
Clinical applications of flow cytometry
An indispensable authoritative text on flow cytometry, the third edition of Flow Cytometry: First Principles is an essential resource for scientists, clinicians, technologists, instructors, and students.
Contents
Setting the Scene
Instrumentation: Into the Black Box
Information: Harnessing the Data
Seeing the Light: Lasers, Fluorochromes, and Filters
Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating
Cells from Within: Intracellular Proteins
Cells from Within: DNA in Life and Death
The Sorting of Cells
Disease and Diagnosis: The Clinical Laboratory
Out of the Mainstream: Research Frontiers
Flowing On: The Future
