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Full Description
Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
Contents
List of Figures, xix List of Color Plates, xxiii List of Tables, xxv List of Protocols, xxvii List of Minireviews, xxix Preface and Acknlowledgments, xxxi Abbreviations, xxxiii About the Companion Website xxxviii 1. Introduction, 1 2. Biology of Cultured Cells, 19 3. Laboratory Design and Layout, 37 4. Equipment and Materials, 51 5. Aseptic Technique, 73 6. Safety, Bioethics, and Validation, 89 7. Culture Vessels and Substrates, 111 8. Defined Media and Supplements, 125 9. Serum-Free Media, 149 10. Preparation and Sterilization, 173 10. Supplementary Material* 11. Primary Culture, 207 11. Supplementary Material* 12. Subculture and Cell Lines, 235 13. Authentication and Validation, 259 14. Microbial Contamination, 289 15. Cryopreservation and Banking, 307 16. Cloning and Selection, 327 16. Supplementary Material* 17. Cell Sorting, 347 18. Cell Line Characterization, 359 19. Differentiation, 383 20. Three ]Dimensional Culture 401 21. Scale ]up and Automation, 427 22. Senescence, Immortalization, and Transformation, 455 23. Quantitation, 483 23 Supplementary Material* 24. Cytotoxicity, 513 24. Supplementary Material* 25. Culture of Specific Cell Types, 531 25. Supplementary Material* 26. Stem Cells, 571 26. Supplementary Material* 27. Training Programs, 591 28. Problem Solving 627 29. In Conclusion, 651 Supplementary Material* Appendix III: Sources of Equipment and Material Appendix IV: Suppliers and Other Resource
