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Full Description
This volume details comprehensive methods for the isolation of pigments from microalgae with emphasis on sustainable and efficient methods. Chapters guide readers through solvent extraction, supercritical fluid extraction, pulsed electric field protocols, and purification methods necessary to produce good quality pigments. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols.
Authoritative and cutting-edge Microalgae-based Pigments: Basic Protocols aims to be comprehensive guide for researchers in the field.
Contents
Synthetic biology approaches for optimizing pigment biosynthesis pathways in microalgae.- Optimization of culture conditions for pigment enhancement from microalgal strains.- Enzymatic hydrolysis and parameter optimization for the recovery of carotenoids from microalgae.- Evaluating the quality, safety and stability of phycobiliproteins from microalgae.- Supercritical carbon-dioxide extraction of astaxanthin from Haematococcus pluvialis.- Optimization of the pulsed electric field-assisted extraction of C-phycocyanin from Spirulina platensis dry biomass.- Purification and characterisation of allophycocyanin from microalgae: As a natural and food grade stable pigments.- Optimization of downstream processing for chlorophyll recovery from microalgal biomass.- Ultrasound-assisted extraction of R-phycoerythrin from Grateloupia turuturu.- Inter-laboratory assessment of HPLC techniques for microalgal pigments: towards standardized analytical procedures.- Pigment Stability and Degradation Under Different Storage and Processing Conditions.- Development and validation of LC-MS analysis of microalgal pigments.- Regulatory protocols and ethical considerations in microalgae pigment trade.
