Full Description
This detailed volume collects cutting-edge experimental protocols and methodological approaches for studying the dynamic interplay between viruses and the host immune system. The book covers a wide range of subjects, including assays to dissect T- and B-cell responses, techniques for analyzing viral escape mutations, and tools to study immune modulation by viral proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Antiviral Adaptive Immunity and Viral Immune Evasion serves not only as a practical guide but also as an inspiration for new lines of inquiry into the complex mechanisms of viral immunity and evasion.
Contents
Brief Introduction to Adaptive Immunity.- Brief Introduction to Antiviral Adaptive Immunity.- The Application of the Organoid Model to Investigating Adaptive Immunity.- Evaluation of Antigen-Specific T-Cell Killing Reactions in Mice by Flow Cytometry.- Immunofluorescence Techniques to Detect Viral Adaptive Immunity and Viral Immune Evasion.- Enzyme-Linked Immunosorbent Assay to Detect Viral Adaptive Immunity and Viral Immune Evasion.- Radioimmunoassay (RIA) to Detect Viral Adaptive Immunity and Viral Immune Evasion.- Detecting Adaptive Immune Cell Function in Viral Infection via the ELISPOT Assay.- Multiplex Fluorescence in Antiviral Adaptive Immune Responses.- Flow Cytometry in Antiviral Immunity and Immune Evasion.- Application of Single-Cell Sequencing Technology in Investigating Antiviral Acquired Immunity and Immune Evasion Mechanisms.- Dynamic Imaging of Acquired Immune Cells in Viral Infection.- Flow Cytometry-Based Protocol for Isolating and Assessing CD8⁺ T-Cells in LCMV-Infected Mice.- Peptide-ELISA for Cross-Genotype Antibody Detection Against the Infectious Bronchitis Virus Spike Protein.- CEK Cell-Based Indirect Immunofluorescence Assay for Infectious Bronchitis Virus Neutralizing Antibodies.- PhIP-Seq Application in the Adaptive Immunity of Viral Infections: Lessons from COVID-19.- EBV-Related Adaptive Immunity in Central Nervous System Demyelinating Diseases.- Peptidome as an Advanced Method in the Exploration of Virus-Induced Adaptive Immunity.- MHC Multimer Technology for the Detection of Virus-Specific Antigen-Specific T-Cells.- Application of a Chemiluminescent Immunoassay in Detecting Acquired Immune Cells During Viral Infection.- Electrochemiluminescence for Detecting Acquired Immune Cells in Viral Infection.- Liquid Chip-Based Functional Analysis of Antiviral-Acquired Immune Responses.- TCR and BCR Repertoires Analysis in Viral Infection.- Biosensor Techniques for Profiling Virus-Induced Adaptive Immune Responses.- Microfluidic Chip-Based Technologies to Monitor Antiviral Adaptive Immune Responses.- Immunoradiometric Assay to Detect Acquired Immune Responses During Viral Infection.- Immuno-PCR Technology to Detect Acquired Immunity During Viral Infection.- Quantitative Monitoring of Virus-Specific Adaptive Immunity via Droplet Digital PCR.- Cytotoxicity and Cell Viability Assays for Viral Infection and Drug Exposure.- Base-Editing-Mediated Viral Gene Mutations to Identify Viral-Specific Cytotoxic T Lymphocytes (CTLs).
