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Full Description
This volume helps readers navigate the entire microbial 'omics research pipeline—from sample collection to data interpretation, and from foundational protocols to cutting-edge applications, with the goal of unifying traditional experimental methods with modern computational tools and emerging technologies. The chapters in this book are organized into five parts. Part One (Foundational Overview) provides a historical context for microbial omics and outlines key methods. Part Two (Core Experimental Methods) focuses on standardized experimental protocols of microbial 'omics research. Part Three (Data Analysis Tools) covers computational tools that transform processed data into meaningful insights. Part Four (Applied 'Omics: From Health to Industry) discusses the broad applicability of microbial 'omics, and Part Five (Cutting-Edge Technologies: AI and Virology) explores the integration of AI and Large Language Models (LLMs) in virology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Microbial 'Omics: Methods and Protocols is a valuable resource for both novice and expert researchers looking to expand their knowledge of microbial 'omics. This book will inspire new discoveries and advance the field for the benefit of humanity and the planet.
Contents
A Brief Review of Microbial Omics: Methods and Perspectives.- Standardized Protocols for Environmental Sample Collection: Minimizing Contamination and Preserving Microbial Community Integrity.- Metagenomic Data Preprocessing and Quality Control.- Metagenomic Assembly and Gene Prediction.- Absolute Quantification of Bacteria in the Microbiome and Its Application.- Network Analysis in Microbiome Research: Methods, Tools, and Applications.- Unlocking Enzyme Discovery: Leveraging Multi-Omics, Machine Learning, and De novo Design.- Omics Approaches to Unraveling the Complexity of the Gut-Lung Axis.- Gut Microbial Metabolism in Colorectal Cancer.- The Role of EBV Infection and Epigenetic Factors in Radioresistance of Colorectal Cancer.- Infection-Associated Microecology and Hepatocellular Carcinoma.- Microbial Omics Analysis for Multi-Species Symbioses in Staple and Traditional Fermented Foods.- Microbial Omics Analysis for Multi-Species Symbioses in Speciality Fermentations and Non-Food Fermentations.- Omics-Based Insights into Polysaccharide Biosynthesis in Macrofungi.- Preparation and Characterization of Metal-Tannic Acid Coated Microorganisms.- Omics Technologies-Driven Research and Applications of Synthetic Biology.- RNA Large Language Models in Virology.- Protein Language Models in Virology: A Review of Advances and Applications.
