Full Description
This new edition explores the innovation and improvement of experimental techniques harnessed to dissect the complex relationship between cancer cells and the immune system of the host. Beginning with spectral and multicolor flow cytometric methods to characterize the immune contexture of different forms of metastases, the book continues with functional assays to study the activation status of immune cells with antitumor properties, bioinformatic approaches of interest for cancer immunology research, techniques for performing epigenetic studies, as well as methods related to translational research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Cancer Immunosurveillance: Methods and Protocols, Second Edition serves as a valuable resource for scientists seeking inspiration for new ideas and research in the field of tumor immunology.
Contents
Characterization of Bone Marrow-Derived Immune Compartment in Murine Brain Metastases by Spectral Flow Cytometry.- Multicolor Flow Cytometry for Immune Characterization of Omental Metastasis in Peritoneal Carcinomatosis Mice Models.- Application of Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-Seq) to Precisely Dissect the Brain Tumor Immune Microenvironment.- Magnetic Bead Based Isolation of CD8+ T Cells from the Mouse Spleen.- Phosphoflow: Evaluation of Protein Phosphorylation Status by Cytofluorimetric Assay.- Evaluation of NF-kB Nuclear Translocation in Natural Killer Cells by Imaging Multispectral Flow Cytometry as a Marker of Anticancer Immune Activation.- A Colony Formation-Based Assay to Evaluate Natural Killer Cell Antitumor Responses In Vitro.- NKT Hybridoma Cell Stimulation Assays to Evaluate Glycolipid Specificity and Processing.- Metabolic Profiling of Tumor and Immune Cells Integrating Seahorse and Flow Cytometry.- Kinetic Assessment of HMGB1 Exodus by Automated Live Cell Imaging.- The Immunogenic Tumor Specific Neoantigen Database (ITSNdb): A Tool for Comprehensive Performance Evaluation of Neoantigen Immunogenicity Predictors.- A Robust Quantitative Multiplex PCR for T-Cell Receptor Repertoire Sequencing from Formalin-Fixed Paraffin-Embedded Samples.- Immune Single-Cell Annotation by Molecular Signature-Based Deconvolution with MIXTURE.- Chromatin Preparation from Frozen Tissues for Chromatin Immunoprecipitation (ChIP) Assays.- Exploring the Cancer Immune Epigenome by ATAC-Seq.- Epigenomic Profiling of Histone Post-Translational Modifications in Frozen Cancer Tissue Using CUT&RUN.- A Luminescence-Based Method for In Vitro Screening of Immunomodulatory Checkpoints and Therapeutics.- Generation of Human Monocyte-Derived Immunogenic Dendritic Cells via mTORC1 and PPARγ Inhibition.- Engineered CAR-NK Cells for Cancer Immunotherapy: Lentiviral-Based CAR Transduction of NK-92 Cells.- Optimal Structural Designs of Trispecific Antibodies to Enhance Therapeutic Efficacy in Solid Tumors and Hematological Malignancies.- Microbiota Transplantation in Tumor Immunology Studies.
