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Full Description
This fully updated volume explores olfactory receptor research as it advances from molecular to system levels. Beginning with chapters on the large ensemble imaging method, the book continues by examining machine learning-related olfaction studies, assays to study odorant receptor activity, olfaction-related behavior techniques, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Olfactory Receptors: Methods and Protocols, Second Edition serves as an ideal reference for newcomers to the olfaction field as well as for experienced professionals looking to master advanced methods.
Contents
In Vivo Two-Photon Imaging of the Olfactory System in Insects.- Calcium Imaging in Olfaction: Non-Rodent Mammalian Species.- Purification of Nuclear and Cytoplasmic RNA from the Mouse Olfactory Epithelium.- Predictive Machine Learning Models for Olfaction.- Combining Machine Learning and Electrophysiology for Insect Odorant Receptor Studies.- Measurement and Analyses of Olfactory Event-Related Potentials.- Chemotaxis in C. elegans: Interrogating the Effects of Odorants on Physiology.- Ex Vivo Multisite Electro-Olfactogram Recordings in Rabbit Neonates.- Image-Based cAMP Sensor Assay for Analyzing Odorant Receptor Activity.- Protein-Protein Interactions of Odorant and G Protein-Coupled Receptors: A BRET Analysis Approach.- Yellow Fluorescent Protein Quenching Assay for Analyzing Odorant Receptor Activity.- Closed Loop Optogenetic Stimulation of the Olfactory Circuit at Different Phases of Theta Oscillations of the Local Field Potential.- Bilateral Optogenetic Stimulation of the Olfactory Bulb of OMP-ChIEF Mice.- Deorphanization of Olfactory Trace Amine-Associated Receptors.- Behavioral Assays in the Study of Olfaction.- AnimalTA: A Step-by-Step Tutorial.
