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Full Description
This fully updated volume presents protocols crucial for contemporary influenza virus research, placing a special emphasis on the latest advancements in imaging technologies, including super-resolution microscopy, cryo-electron tomography (cryo-ET), freeze replica electron microscopy, and high-speed atomic force microscopy (AFM). In addition to covering traditional methods like virus purification, this edition introduces innovative approaches for visualizing molecular interactions between viral proteins and host proteins, methods for developing physiological cell culture models and animal models, as well as concepts concerning the prediction of viral evolution and the utilization of artificial intelligence in infection biology research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Influenza Virus: Methods and Protocols, Second Edition serves as an ideal guide for researchers looking to deepen our understanding of the intricate interplay between viruses and host cells.
Contents
Understanding Influenza.- Purification of Influenza Virions.- Animal Models in Influenza Research.- Live-Cell Single-Molecule Imaging of Influenza A Virus Receptor Interaction.- Monitoring Influenza A Virus Entry Using Quantitative Fluorescence Microscopy.- Fluorescence Loss After Photoactivation (FRAPh): A Pulse-Chase Cellular Assay for Understanding Kinetics and Dynamics of Viral Inclusions.- Purification and Ultramicroscopic Observation of the Influenza A Virus Ribonucleoprotein Complex.- Room Temperature Transmission Electron Microscopy of Influenza A Virus Samples.- Sample Preparation for Cryo-Electron Tomography of Influenza A Virus and Infected Cells.- Advanced Freeze-Replica Methods for Virus Cell Biology.- Mapping Binding Sites of Nucleoprotein Within the Influenza Virus RNA Genome.- Human Induced Pluripotent Stem Cell-Based Alveolus-on-Chip Model to Study Influenza Virus A Infection.- Nasal Muco-Ciliary Epithelial Cell Culture Models for Studying Viral Infections.- Concepts and Methods for Predicting Viral Evolution.- Artificial Intelligence Methods in Infection Biology Research.
