Hepatitis B Virus : Methods and Protocols (Methods in Molecular Biology) （2ND）

個数：

Hepatitis B Virus : Methods and Protocols (Methods in Molecular Biology) （2ND）

Guo, Haitao (EDT)/ Cuconati, Andrea (EDT)

ウェブストア価格 ¥52,445（本体¥47,678）
Springer-Verlag New York Inc.（2024/08発売）
外貨定価 US$ 249.99
ポイント 476pt

提携先の海外書籍取次会社に在庫がございます。通常3週間で発送いたします。
【重要ご説明事項】
1. 納期遅延や、ご入手不能となる場合が若干ございます。
2. 複数冊ご注文の場合は、ご注文数量が揃ってからまとめて発送いたします。
3. 美品のご指定は承りかねます。

●3Dセキュア導入とクレジットカードによるお支払いについて

【入荷遅延について】
世界情勢の影響により、海外からお取り寄せとなる洋書・洋古書の入荷が、表示している標準的な納期よりも遅延する場合がございます。
おそれいりますが、あらかじめご了承くださいますようお願い申し上げます。

◆画像の表紙や帯等は実物とは異なる場合があります。

◆ウェブストアでの洋書販売価格は、弊社店舗等での販売価格とは異なります。
また、洋書販売価格は、ご注文確定時点での日本円価格となります。
ご注文確定後に、同じ洋書の販売価格が変動しても、それは反映されません。

製本 Hardcover:ハードカバー版／ページ数 216 p.
言語 ENG
商品コード 9781071640265

Full Description

This detailed new edition collects state-of-the-art methods in the fields of hepatitis B virus (HBV) molecular biology and immunology. The book features techniques for cell culture, epigenetic regulation of cccDNA transcription, epitranscriptomic modification of HBV RNA, HBV pregenomic RNA and subviral particle morphogenesis, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Hepatitis B Virus: Methods and Protocols, Second Edition serves as an ideal guide for researchers pursuing a cure for this chronic disease.

Stem Cell-Derived Hepatocyte-Like Cells for Hepatitis B Virus Infection.- Primary Duck Hepatocyte Culture and Duck Hepatitis B Virus Infection Model.- Hepatitis B Virus Covalently Closed Circular DNA Chromatin Immunoprecipitation Assay.- Analysis of HBV cccDNA Minichromosome Accessibility by MNase-qPCR and High Throughput Sequencing.- Circular Chromosome Conformation Capture (4C-Seq) Analysis of HBV-Host Chromosome DNA Interactome.- Analysis of N6-Methyladenosine Modification of HBV RNA by Methylated RNA Immunoprecipitation.- Structural Analysis of the Hepatitis B Virus RNA Encapsidation Signal ɛ by Selective 2'-Hydroxyl Acylation Analyzed by Primer Extension (SHAPE).- A Particle Gel Assay for Detection of Intracellular Hepatitis B Virus Subviral Particles in Cultured Cells.- Detection of Hepatitis B Virus Covalently Closed Circular DNA and Intermediates in Its Formation.- Rapid and Reliable Protein-Free HBV DNA Extraction and Sensitive Branched DNA Southern Blot Assay.- A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture.- A Chromogenic In Situ Hybridization (CISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Liver Tissue.- Detection and Quantification of HBV Transcripts by Full-Length 5'RACE-PCR.- One-Step Reverse Transcriptase qPCR Method for Serum Hepatitis B Virus RNA Quantification.- Method for Quantitative HDV RNA Detection: I, Manual Workflow (Serum and Liver Tissue) and II, Fully Automated High Throughput Workflow for Diagnostic Use.- The Cre/loxP-Based Recombinant HBV cccDNA System In Vitro and In Vivo.- Modeling HBV Infection and Therapy in Immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (FAH) Knockout Mice with Human Chimeric Liver.- Production and Purification of Adeno-Associated Viral Vectors for the Development of Immune-Competent Mouse Models of Persistent Hepatitis B Virus Replication.- Peptide-Loaded HLA Class I Tetramer-Associated Magnetic Bead-Based Enrichment of HBV-Specific CD8+ T Cells.- Optimized Ex Vivo Fluorospot Assay to Measure Multi-Functional HBV-Specific T Cell Frequencies in Chronic Hepatitis B Patients.- Ex Vivo Fluorescent Labeling of HBV-Specific B Cells in Chronic Hepatitis B Patients.- In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV Ribonuclease H Inhibitors.