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Full Description
This second edition guides readers through experimental and computational techniques on the study of tissue morphogenesis, with a specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.
Contents
Isolation, culture, and phenotypic analysis of murine lung organoids.- Using 3-dimensional cultures to propagate genetically modified lung organoids.- High-throughput assembly of compositionally controlled 3D cell communities for developmental engineering.- Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors.- Engineered heart tissues for standard 96-well tissue culture plates.- Building biomaterials to mimic 3D cell-cell junctions.- Bioprinting cell-laden hydrogels for studies of epithelial tissue morphogenesis.- Genome-wide profiling of cis-regulatory elements in mammalian skin.- Multi-labeling live imaging to quantitate gene expression dynamics during Drosophila embryonic development.- PyJAMAS: Python-based analysis of biological images.- Mechanical compression of Drosophila embryos using rapid fabrication microfluidic devices.- Lumen pressure modulation in chicken embryos.- Quantifying spatial patterns of tissue stiffness within the embryonic mouse kidney.- Evaluating planar cell polarity in the developing mouse epidermis.- Purification of planarian stem cells using a Draq5-based FACS approach.- Arabidopsis root microbiome microfluidic (ARMM) device for imaging bacterial colonization and morphogenesis of Arabidopsis roots.
