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Full Description
This detailed volume explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microfluidics Diagnostics: Methods and Protocols serves as an ideal guide for researchers investigating how microfluidics can shape the future of diagnosis.
Contents
Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids.- DNA Sizing with pg/mL Sensitivity for Cell-Free DNA Analysis with the mLAS Technology.- On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples.- Isolation of Extracellular Vesicles from Cell Culture and Blood through Nano-Targeted DLD Microfluidic Device.- Microchip for immunomagnetic sorting of Circulating Tumor Cells (CTCs).- Enhancing the Efficiency of Conventional Surface Immunoassays within Standard Labware Using Microscale Flows.- Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis.- Microfluidic Cartridge for Bead-Based Affinity Assays.- A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level.- Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets.- A Thermoplastic Microsystem to Perform Antibiotic Susceptibility Testing by Monitoring Oxygen Consumption.- An Immiscible-Phase Filtration Device for Isolation, Amplification, and Detection of Nucleic Acids for Clinical Diagnostics.- Microfluidic 3D Cytotoxic Assay.- Evaluating Nanoparticles Penetration by a New Microfluidic Hydrogel-Based Approach.- Selective Targeting of Tumor Cells in a Microfluidic Tumor Model with Multiple Cell Types.
