Full Description
This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts and written in the highly successful Methods in Molecular Biology series format, each chapter includes introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and valuable tips for troubleshooting and avoiding known pitfalls.
Whether you are a seasoned scientist or a newcomer, Bacteriophage Therapy: From Lab to Clinical Practice, Second Edition provides essential tools and knowledge to advance bacteriophage research and its translation into innovative medicinal products and clinical therapies.
Contents
Isolation of Bacteriophages for Clinically Relevant Bacteria.- Observation of Bacteriophage Ultrastructure by Cryo-electron Microscopy.- Bacteriophage Taxonomy: A Continually Evolving Discipline.- Guidelines to Compose an Ideal Bacteriophage Cocktail.- Rapid Bench to Bedside Therapeutic Bacteriophage Production.- Bacteriophage Production in Compliance with Regulatory Requirements.- Nano/micro formulations for bacteriophage delivery.- Phage-Host Interaction Analysis using Flow Cytometry.- Bacteriophage control of infectious biofilms.- Studying bacteriophage efficacy using a zebrafish model.- Use of Galleria mellonella as an animal model for studying the antimicrobial activity of bacteriophages with potential use in phage therapy.- Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-specific Antibodies.- Bacteriophage Treatment of Infected Diabetic Foot Ulcers.- A Review of Phage Therapy for Bone and Joint Infections.- Successful Use of Phage and Antibiotics Therapy for the Eradication of Two Bacterial Pathogens from the Respiratory Tract of an Infant.- Genetic engineering and rebooting of bacteriophages in L-form bacteria.- Synthetic Biology to Engineer Bacteriophage Genomes.- Genetic engineering of therapeutic phages using Type III CRISPR-Cas systems.- Robust and reproducible protocol for phage genome "rebooting" using transformation-associated recombination (TAR) cloning into yeast centromeric plasmid.
