Full Description
This fully updated volume explores recently improved avenues to study urothelial carcinomas. Beginning with several novel chapters on molecular characterization and urothelial carcinogenesis, the book continues with sections on cellular and animal models, biomarkers, and approaches for targeted therapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Urothelial Carcinoma: Methods and Protocols, Second Edition serves as a valuable resource to further increase our knowledge on urothelial carcinoma and also to aid research on numerous other cancers.
Contents
Scoring Systems for Immunohistochemistry in Urothelial Carcinoma.- A Panel-Based Method for the Reproduction of Distinct Molecular Subtype Classifications of Muscle-Invasive Urothelial Bladder Cancer.- Analysis of Mutational Signatures Using the mutSignatures R Library.- A Drug Repurposing Pipeline Based on Bladder Cancer Integrated Proteotranscriptomics Signatures.- Characterization of Native COMPASS Complex in Urothelial Carcinoma Cells by Size Exclusion Chromatography.- Reconstructing Phylogenetic Relationship in Bladder Cancer: A Methodological Overview.- Using Sister Chromatid Exchange Assay to Detect Homologous Recombination Deficiency in Epigenetically Deregulated Urothelial Carcinoma Cells.- Identification of STAG2 Mutant Bladder Cancers by Immunohistochemistry.- Genome-Wide CRISPR Screening for the Identification of Therapy-Resistance-Associated Genes in Urothelial Carcinoma.- Tissue Slice Culture and Analysis of Tumor-Associated Hyaluronan in Urothelial Carcinoma.- NGS-Based Tumor-Informed Analysis of Circulating Tumor DNA.- Considering the Effects of Modern Point-of-Care Urine Biomarker Assays in Follow-Up of Patients with High-Risk Non-Muscle Invasive Bladder Cancer.- Simplex Droplet Digital PCR Assays for the Detection of TERT Promoter Mutations in Urine Samples for the Non-Invasive Diagnosis of Urothelial Cancer.- Predictive Biomarkers of Response to Neoadjuvant Therapy in Muscle Invasive Bladder Cancer.- Assessment of PD-L1 Status in Urothelial Cancer.- Epigenetic Priming and Development of New Combination Therapy Approaches.- Evaluation of FGFR Alteration Status in Urothelial Tumors.- Antibody-Drug-Conjugates (ADC): A Novel Treatment Option in Urothelial Carcinoma.- Intravesical Infusion of Oncolytic Virus CG0070 in the Treatment of Bladder Cancer.- Analysis of ICAM-1 Expression on Bladder Carcinoma Cell Lines and Infectivity and Oncolysisby Coxsackie Virus A21.
