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Full Description
This second edition provides new and updated tools for studying protein-carbohydrate interactions ranging from traditional biochemical methods to state-of-the-art techniques. This book focuses on four different research themes detailing methods for screening and quantifying CAZyme activity, investigating the interactions between proteins, carbohydrate ligands, methods for the visualization of carbohydrates, protein-carbohydrate complexes, structural and "omic" approaches for studying systems of CAZymes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Carbohydrate- Protein Interactions: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers inthe field.
Contents
A low-volume, parallel copper-bicinchoninic acid (BCA) assay for glycoside hydrolases.- Measuring enzyme kinetics of glycoside hydrolases using the 3,5-dinitrosalicylic acid assay.- Analyzing activities of lytic polysaccharide monooxygenases by liquid chromatography and mass spectrometry.- Carbohydrate depolymerization by intricate cellulosomal systems.- Biomechanical weakening of paper and plant cell walls by bacterial expansins.- Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions.- Quantifying CBM carbohydrate interactions using microscale thermophoresis.- Qualitative and quantitative characterization of protein-carbohydrate interactions by NMR spectroscopy.- Isothermal Titration Calorimetry for quantification of protein-carbohydrate interactions.- Surface plasmon resonance analysis for quantifying protein-carbohydrate interactions.- Bioinspired polymer assemblies of plant cell walls for measuring protein-carbohydrate interactions by FRAP.- CBMs as probes to explore plant cell wall heterogeneity using immunocytochemistry.- Flow Cytometry-based Detection of Siglec Ligands.-Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.- Analysis of complex carbohydrate composition in plant cell wall using Fourier transformed mid-infrared spectroscopy.- Separation and Visualisation of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis.- A rapid protocol for preparing 8-aminopyrene-1,3,6-trisulfonate-labelled glycans for capillary electrophoresis-based enzyme assays.- Visualization of Carbohydrate Uptake using Fluorescent Polysaccharides.- Long-read Metagenomics and CAZyme discovery.- Identification of genes involved in the degradation of lignocellulose using comparative transcriptomics.- Isolation and preparation of extracellular proteins from lignocellulose degrading fungi for comparative proteomic studies using mass spectrometry.
