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Full Description
This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell function and are key to further development of therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Hematopoietic Stem Cells: Methods and Protocols serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it.
Contents
The Factory of Blood Production: Hematopoietic Stem Cells.- Detection of DNA Damage in Hematopoietic Stem Cells.- Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation.- CRISPR Gene Editing of Hematopoietic Stem and Progenitor Cells.- Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cells.- Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells.- Isolation and Characterization of Fetal Liver Hematopoietic Stem Cells.- Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype.- Hematopoietic Stem Cell Identification Post-Radiation.- Intravital Microscopy for Hematopoietic Studies.- Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biology.- MSC and HSPC Co-Culture: Mimicking Ex Vivo Bone Marrow Niche.- Isolation of Thymus Stromal Cells from Human and Murine Tissue.- Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantation.- Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish.- Establishing a Murine Model of the Hematopoietic Acute Radiation Syndrome.- Purinergic Signaling and Its Role in Mobilization of Bone Marrow Stem Cells.
