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Full Description
This updated volume explores Candida and candidiasis methods, useful to a wide variety of Candida scientists including those new to the field. Beginning with a section on the Candida genome, the book continues by covering techniques for antifungal discovery and studying resistance, pathogenesis and virulence, communities, as well as immunity. Biofilm protocols are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Candida Species: Methods and Protocols, Second Edition serves as an ideal guide for researchers working to further our understanding of this family of infective fungi.
Contents
CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans.- Plasmid-Based CRISPR-Cas9 Editing in Multiple Candida Species.- A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans.- How to Use the Candida Genome Database.- Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata.- Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans.- Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance.- Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS).- High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis.- Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous Saccharomyces cerevisiae Mutant Library.- Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans.- In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin.- Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors.- Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy.- A Simple Method for Growth of Candida albicans Biofilms under Continuous Media Flow and for Recovery of Biofilm Dispersed Cells.- A Simple 96 Well Plate-Based Method for Development of Candida Biofilms under Static Conditions.- Comparative Analysis of the Fitness of Candida albicans Strains during Colonization of the Mice Gastrointestinal Tract.- Detection and Identification of Candida auris from Clinical Skin Swabs.- Production and Isolation of the Candida Species Biofilm Extracellular Matrix.- Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model.- Analysis of Gut Microbiome Using Fecal Samples.- Measurements of Treg Cell Induction by Candida albicans DNA Using Flow Cytometry.- Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.- Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans.- Understanding the Protective Role of IL-17 during Oropharyngeal Candidiasis.
