Full Description
This volume provides detailed protocols for the identification and understanding of vasculogenic mimicry process in vitro and in vivo, in addition to protocols for microscopy and histology. Chapters guide readers through different materials, commercial and homemade scaffolds, Matrigel, cancer spheroids, 3D tissue constructs, vasculogenic processes, and mathematical model building. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Vasculogenic Mimicry: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Contents
Vasculogenic Mimicry - An Overview.- In vitro Models to Study Angiogenesis and Vasculature.- In vitro Tube Formation Assays in Matrigel.- Vasculogenic mimicry in a 3D model in vitro.- Cancer Cell Spheroids as a 3D Model for Exploring the Pathobiology of Vasculogenic Mimicry.- A Three-Dimensional Culture-Based Assay to Detect Early Stages of Vasculogenic Mimicry in Ovarian Cancer Cells.- Co-Immunoprecipitation of Protein Complexes from Different Subcellular Compartments in Vasculogenic Mimicry Studies.- In vivo Models to Evaluate Antitumor Drugs Effect on Vasculogenic Mimicry.- Patient derived xenograft models for studying vascular mimicry in melanoma in vivo.- Histological evaluation of long-term collagen I culture.- Histochemical Staining of Vasculogenic Mimicry.- Identification of Vasculogenic Mimicry in Histological Samples.- Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry in vitro.- Immunohistochemistry for VM markers.- Confocal laser microscopy for VM analysis with DAPI and phalloidin staining.- Building Mathematical Models for Vascular Growth and Inhibition.
