Full Description
This detailed book explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields.
Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.
Contents
Requirements and Preparedness for Attending a Viral Disease Outbreak in Pig Farms.- Collection of Samples, Their Preservation, and Transportation.- Methods for Quantification of Viruses.- Protocols for Isolation of Genetic Materials from RNA Viruses.- Multiplex PCR for Diagnosis of Porcine Diseases.- Protocols for Isolation of Plasmid DNA.- Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases.- RNA-PAGE-Based Diagnosis of Viral Diseases.- Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases.- Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases.- Aptamers as Diagnostic Markers for Viral Infections of Veterinary Importance.- Antibody-Based Sensors for Pathogen Detection.- Lateral Flow Assay for Diagnosis of Pig Viral Diseases.- Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases.- Protocols for Immunofluorescence Techniques.- Polymerase Spiral Reaction (PSR) for the Diagnosis of Porcine Viral Diseases.- Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases.- Cell Culture System for Porcine Virus Isolation and Propagation.- An Overview of Mouse Monoclonal Antibody Production.- Nucleic Acid Hybridization Techniques for Viral Disease Diagnosis: A Detailed Perspective.- Ligase Detection Reaction-Fluorescent Microsphere Assay.- ELISA as a Diagnostic Weapon.- SDS-PAGE and Western Blotting: Basic Principles and Protocol.- Immune Assays as Diagnostic for Pig Viral Diseases.- Production of Virus-Like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis.- Good Laboratory Practices and Biosafety Containments in a Virology Laboratory.
