Full Description
This volume provides methods and techniques to further the study of cancer immunoprevention. Chapters describe tumor-associated antigens, cancer immune-preventive vaccines, generation of TILs, development of monoclonal antibodies, immunoprofiling technologies, tissue multispectral imaging techniques, mass cytometry on suspensions, mutiparametric flow cytometry, genomic expression analysis, and proteomic profiling of tumor microenvironment cell populations and metabolic assessment through novel imaging technologies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Cancer Immunoprevention: Methods and Protocol aims to further understanding, development of interventional active strategies, and immune-interception of cancer.
Contents
Cancer Immunoprevention: General Concepts.- Targets and Strategies for Cancer Immunoprevention.- Discovery of targets for cancer immunoprevention.- Antigen pulsed exosome vaccines for Immunoprevention.- Isolation and Maintenance of Tumor-Infiltrating Lymphocytes for Translational & Clinical applications: established methods and new developments.- Monoclonal Antibodies Generation: Updates and Protocols on Hybridoma Technology.- Transcriptomic-Assisted Immune and Neoantigen Profiling in Premalignancy.- Assessment of the Murine Tumor Microenvironment by Multiplex Immunofluorescence.- Cytometry of Mass for Murine Immunoprofiling.- Interrogating the tumor microenvironment by deep tissue immunoprofiling.- Proteomic profiling of the tumor microenvironment.- Metabolic Imaging using Hyperpolarization for Assessment of Premalignancy.- Murine models for lineage tracing cancer initiating cells.- Murine Model of Obesity-Induced Cancer.
