Full Description
This detailed volume explores numerous methods used in basic science laboratories to characterize cancer-related biomarkers, vital for better managing cancer burden, including cancer risk assessment, cancer diagnosis, determining cancer progression, and therapeutic response. From a radiography method to an examination of single-cell RNA-seq and computational analysis tools in cancer research, this book delves into many techniques that could provide valuable molecular information about the tumor and its microenvironment components. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cancer Biomarkers: Methods and Protocols offers researchers multiple helpful ways to study cancer-associated molecular biomarkers.
Contents
A Method of Bone-Metastatic Tumor Progression Assessment in Mice Using Longitudinal Radiography.- Optical Imaging of Matrix Metalloproteinases Activity in Prostate Tumors in Mice.- Method to Development of PET Radiopharmaceutical for Cancer Imaging.- PET Use in Cancer Diagnosis, Treatment, and Prognosis.- Immunofluorescence-Based Method to Assess Cancer Biomarker in the Hypoxic Region of the Tumor.- Zebrafish Xenograft Model to Study Human Cancer.- Assessing Oligomerization Status of Mitochondrial OXPHOS Complexes via Blue Native Page.- In Vitro Cell Impedance Assay to Examine Antigen-Specific T-Cell-Mediated Melanoma Cell Killing to Support Cancer Immunotherapy Drug Discovery.- Methods to Detect Nitric Oxide and Reactive Nitrogen Species in Biological Sample.- Study of Rotary Cell Culture System Induced Microgravity Effects on Cancer Biomarkers.- A Calcium Imaging Approach to Measure Functional Sensitivity of Neurons.- Zymography and Reverse Zymography for Testing Proteases and TheirInhibitors.- Method of Preparation of Cigarette Smoke Extract to Assess Lung Cancer-Associated Changes in Airway Epithelial Cells.- Air-Liquid Interface Culture Model to Study Lung Cancer-Associated Cellular and Molecular Changes.- Co-Immunoprecipitation-Blotting: Analysis of Protein-Protein Interactions.- Methods to Assess Oxidative DNA Base Damage Repair of Apurinic/Apyrimidinic (AP) Sites Using Radioactive and Non-Radioactive Oligonucleotide-Based Assays.- Determining the Size Distribution and Integrity of Extracellular Vesicles by Dynamic Light Scattering.- Characterization of Exosomal Surface Proteins by Immunogold Labeling.- Scanning Electron Microscopy of Giant Cells from Giant Cell Tumor of Bone.- Raman Microscopy Techniques to Study Lipid Droplet Composition in Cancer Cells.- Surface Plasmon Resonance, a Novel Technique for Sensing Cancer Biomarker: Folate Receptor and Nanoparticles Interface.- Characterization of Tobacco Microbiome by Metagenomics Approach.- Single-CellRNA-Seq Technologies and Computational Analysis Tools: Application in Cancer Research.
