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Full Description
This second edition provides new and updated chapters detailing recent advances in MYC research and current techniques. Chapters guide readers through protocols on how to express and purify MYC protein, X-ray crystallography, NMR, techniques to study how MYC is modified, apoptosis, senescence, proliferation, metabolic changes, translation, tumorigenesis,reprogramming, and clinical application of MYC studies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Myc Gene: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Contents
An "-omycs" Toolbox to Work with MYC.- Methods of Expression, Purification and Preparation of the c-Myc b-HLH-LZ for its Biophysical Characterization.- Biophysical and Structural Methods to Study the bHLHzip Region of Human c-MYC.- Identifying and Validating MYC:protein Interactors in Pursuit of Novel anti-MYC Therapies.- Detection of Post-Translational Modifications on MYC.- MYC Analysis in Cancer and Evolution.- Genome-wide analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing.- Analysis of Myc Chromatin Binding by Calibrated ChIP-seq Approach.- A High-Throughput Chromatin Immunoprecipitation Sequencing Approach to Study the Role of MYC on the Epigenetic Landscape.- Methods for Determining Myc-induced Apoptosis.- Measuring MYC-mediated Metabolism in Tumorigenesis.- Methods to study Myc-regulated Cellular Senescence - An Update.- Examining Myc-Dependent Translation Changes in Cellular Homeostasis and Cancer.- A Versatile in vivo System to Study Myc in Cell Reprogramming.- Using Flow Cytometry to Study Myc's Role in Shaping the Tumor Immune Microenvironment.- Generation of a Tetracycline Regulated Mouse Model of MYC-Induced T-Cell Acute Lymphoblastic Leukemia.- Chromogenic in Situ Hybridization (CISH) as a Method for Detection of C-Myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue: An Update.- MYC Copy Number Detection in Clinical Samples using a Digital DNA-Hybridization and Detection Method.- Cell-Based Methods for the Identification of Myc-Inhibitory Small Molecules.
