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Full Description
This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results.
Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to furtherunderstand these essential proteins and their many biological roles.
Contents
Expression and Purification of Human Mitochondrial Intramembrane Protease PARL.- Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies.- Biochemical Characterization of GPCR-G Protein Complex Formation.- Electrophysiological Approaches for the Study of Ion Channel Function.- Isothermal Titration Calorimetry of Membrane Proteins.- Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level.- Structure Determination of Membrane Proteins Using X-Ray Crystallography.- Studying Membrane Protein Structures by MicroED.- Single-Particle Cryo-EM of Membrane Proteins.- Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy.- NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel.- Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering.- Preparing MembraneProteins for Simulation Using CHARMM-GUI.- Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide.- Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins.- Molecular Dynamics-Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions.- Concepts, Practices, and Interactive Tutorial for Allosteric Network Analysis of Molecular Dynamics Simulations.- Large Scale Molecular Dynamics Simulations of Cellular Compartments.
