Full Description
This detailed book explores protocols for a wide array of preclinical pharmacology and toxicology evaluations to be applied to chemical drugs and their development through in vitro, involving tissues and cell lines, and in vivo models, using animals as experimental systems, utilized to conduct pharmacological research. Written for the Springer Protocols Handbooks series, the methodologies included in this collection have been standardized by the authors through extensive use in the lab so that they are ready to be applied in the labs of readers around the world.
Authoritative and practical, Bioassays in Experimental and Preclinical Pharmacology aims to assist undergraduate and postgraduate students, research scholars, scientists, and other academicians performing research in the vital field of drug discovery.
Contents
Overview.- Cell Culture Assays.- Phytochemical Analysis.- Preclinical Drug Dose Calculation.- Toxicology Studies (In Vitro and In Vivo).- Standard Procedure for Anesthesia in Preclinical Experiments.- General Considerations and Collection of Animal Blood.- Animal Experiments on the Cardiovascular System.- Animal Experiments on Ulcerative Colitis.- Experiments on Anti-Bacterial Activities.- Experiments on Anti-Fungal Activities.- Animal Experiment on Wound Healing Activity.- Animal Experiments of Anti-inflammatory Activities.- Animal Experiment on Anti-Nociceptive Activities.- Experiments on Anti-Oxidant Activities.- Animal Experiments on Hepatoprotective Activities.- Animal Experiments on Anti-Diarrheal Activities.- Animal Experiments on Central Nervous System (CNS).- Experiments on Anti-Cancer Activities (In Vitro and In Vivo).- Animal Experiments on Anti-Diabetic Activities.- Animal Experiments on Gastric Ulcer.- Protein Extraction and Western Blot Analysis.- Gel Electrophoresis and PCR Amplification.- Molecular Docking Methods for Drug Design.
