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Full Description
This volume provides basic and cutting-edge methods and protocols to study the major characteristics of eukaryotic cells. Chapters detail the different pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins involved in exocytosis, specific protocols for exocytosis in specialized cells such as neutrophils or neuroendocrine cells, as well as secretion of exosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and clearly written, Exocytosis and Endocytosis: Methods and Protocols is a valuable resource for researchers in the fields of cell biology, neurology, immunology, oncology, and those interested in studying protein trafficking and signal regulation.
Contents
Stoichiometry of Receptors at the Plasma Membrane during Their Endocytosis using Total Internal Reflection Fluorescent (TIRF) Microscopy Live Imaging and Single Molecule Tracking.- Measuring Endocytosis During Proliferative Cell Quiescence.- Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution In Adrenal Chromaffin Cells.- Quantitative Methods to Study Endocytosis and Retrograde Transport of Cargo Proteins.- High-content Drug Discovery Screening of Endocytosis Pathways.- Methods for Monitoring Endocytosis in Astrocytes.- Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent Dextrans.- Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretion.- High-throughput Screening for Insulin Secretion Modulators.- Different Approaches to Record Human Sperm Exocytosis.- Bovine Chromaffin Cells: Culture and Fluorescent Assay for Secretion.- Measurement of Exocytosis in Genetically Manipulated Mast Cells.- Super-Resolution Microscopy and Particle Tracking Approaches for the Study of Vesicular Trafficking in Primary Neutrophils.- An Approach to Monitor Exocytosis in White Adipocytes.- Amperometry in Single Cells and Tissue.- Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging In Mouse Adrenal Chromaffin Cells.- RUSH-synchronized Secretion to Measure Local Exocytosis.- Combining Single Molecule Super-Resolution Imaging Techniques to Unravel the Nanoscale Organization of the Presynapse.- Induction of Ca2+ Dependent Exocytotic Processes by Laser Ablation of Endothelial Cells.- Transmission Electron Microscopy and Tomography on Plasma Membrane Sheets to Study Secretory Docking.- Spatial and Temporal Aspects 0f Exocytosis Studied on the Isolated Plasma Membranes.
