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Full Description
This detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications. The collection discusses vectors other than the traditional viral and non-viral systems and explores ideas for blending the best features of bacteria, nanoparticles, peptides, and hybrid systems for the delivery of biomaterials into cells, as well as a chapter on the application of shotgun proteomics and mass spectrometry as a tool to analyze the proteomic profile changes in cells that result from these interventions. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,Bio-Carrier Vectors: Methods and Protocols serves as an ideal guide for researchers working toward harnessing the power of multiple vectors in gene and drug delivery.
Contents
Improving E. coli Bactofection by Expression of Bacteriophage ΦX174 Gene E.- Improved DNA Delivery Efficiency of Bacterial Vectors by Co-Delivery with Exogenous Lipid and Antimicrobial Reagents.- Visualization of Bacteria-Mediated Gene Delivery Using High Resolution Electron and Confocal Microscopy.- Assessing Nucleic Acid:Cationic Nanoparticle Interaction for Gene Delivery.- Catanionic Hybrid Lipid Nanovesicles for Improved Bioavailability and Efficacy of Chemotherapeutic Drugs.- Magnetic Nanoparticles as a Carrier of dsRNA for Gene Therapy.- Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells through Protein Transduction of Transcription Factors.- Cardiac Targeting Peptide: From Identification to Validation to Mechanism of Transduction.- Lactoferricin-Derived L5a Cell-Penetrating Peptide for Delivery of DNA into Cells.- Delivery of Peptide Nucleic Acids Using an Argininocalix[4]arene as Vector.- Cell-Derived Nanovesicles as Exosome-Mimetics forDrug Delivery Purposes: Uses and Recommendations.- Hydrogel Beads of Natural Polymers as a Potential Vehicle for Colon-Targeted Drug Delivery.- Scaffold-Based Delivery of CRISPR/Cas9 Ribonucleoproteins for Genome Editing.- Correction of Hemoglobin E/Beta-Thalassemia Patient-Derived iPSCs Using CRISPR/Cas9.- CRISPR/Cas9-Mediated GFP Reporter Knock-In in K562 and Raji Cell Lines for Tracking Immune Cell Killing Assay.- Shotgun Proteomics and Mass Spectrometry as a Tool for Protein Identification and Profiling of Bio-Carrier-Based Therapeutics on Human Cancer Cells.
