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Full Description
This updated edition emphasizes the benefits of structural genomics to the wider structural research community with a diverse range of applicable methods. The book opens with an extensive section on protein production, including new methodologies for membrane and metal-binding proteins, as well as high-throughput protein production and screening. It continues with sections on structural analyses and data management, as well as modeling, simulation, and visualization. Overall, the spectrum of topics reflects the trend towards tackling more diverse challenges of studying macromolecular machineries and complexes. Written for the highly successful Methods in Molecular Biology series, chapters include the type of detail and key implementation advice to ensure successful results.
Authoritative and up-to-date, Structural Genomics: General Applications, Second Edition serves as a vital guide to structural biologists in small to medium-sized laboratories seeking to better utilize this powerful area of study.
Chapter 11 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. For further details see license information in the chapter.
Contents
High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis.- Rational Design and Construction of Active-Site Labeled Enzymes.- Screening and Production of Recombinant Human Proteins: Ligation Independent Cloning.- Screening and Production of Recombinant Human Proteins: Protein Production in E. coli.- Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells.- Expression Screening of Human Integral Membrane Proteins Using BacMam.- High-Throughput Expression Screening in Mammalian Suspension Cells.- In Vitro Production of Perdeuterated Proteins in H2O for Biomolecular NMR Studies.- Minimizing Heterogeneity of Protein Samples for Metal Transporter Proteins Using SAXS and Metal Radioisotopes.- Hydrogen/Deuterium Exchange-Mass Spectrometry for Probing Changes in Conformation and Dynamics of Proteins.- BeStSel: From Secondary Structure Analysis to Protein Fold Prediction by Circular Dichroism Spectroscopy.- Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb.- State-of-the-Art Data Management: Improving the Reproducibility, Consistency, and Traceability of Structural Biology and In Vitro Biochemical Experiments.- Protein Structure Modeling with MODELLER.- Parameterization of a Dioxygen Binding Metal Site Using the MCPB.py Program.- Parameterization of Large Ligands for Gromacs Molecular Dynamics Simulation with LigParGen.- Simulation of Proteins Modified with a Fluorescent Label.- Protocol for Simulations of PEGylated Proteins with Martini 3.- Molecular Data Visualization on Mobile Devices: A Quick Starter's Guide.- Molecular Data Visualization with Augmented Reality (AR) on Mobile Devices.
