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Full Description
This comprehensive laboratory manual describes the various protocols involved in Actinobacterial research. The content is divided into fifteen major sections, each of which is further divided into sub-sections describing the respective aim, principles, materials & methods, protocol, expected results and diagrams. Readers will find essential protocols for e.g. sample collection, isolation, characterization, analysis, profiling and evaluation of Actinobacteria for various applications. Gathering all relevant protocols concerning Actinobacteria, and written by a team of experienced Actinobacterial researchers, it is the first book of its kind.
Contents
Chapter 1. Sample collection, isolation and diversity of actinobacteria.- Chapter 2. Dereplication and ex situ conservation of actinobacteria.- Chapter 3. Characterization and identification of actinobacteria.- Chapter 4. Screening of actinobacteria for biological activities.- Chapter 5. Production of bioproducts from actinobacteria.- Chapter 6. Eliciting cryptic metabolite production from actinobacteria.- Chapter 7. Metabolite profiling of actinobacterial metabolites.- Chapter 8. Bioassay guided isolation and characterization of metabolites from actinobacteria.- Chapter 9. Evaluation of actinobacteria for environmental applications.- Chapter 10. Evaluation of actinobacteria for agricultural applications.- Chapter 11. Evaluation of actinobacteria for aquaculture applications.- Chapter 12. Evaluation of actinobacteria for nanoparticle synthesis.- Chapter 13. Determination of cytotoxicity of actinobacterial extracts & metabolites.- Chapter 14. Evaluation of actinobacteria for bio energyapplications.
