Full Description
This book provides a comprehensive overview of the application of microfluidic technology in the analysis and manipulation of mammalian cells. It introduces the fundamental principles of microfluidics and explores their use in measuring and controlling the biochemical and biophysical properties of cells for disease diagnosis and therapeutic development.
Microfluidic Technologies for Cell Analysis and Beyond centers on the comprehensive analysis of cells using microfluidic systems. It discusses microfluidics and their applications in the biochemical and biophysical analysis of both single cells and cell populations. Accessible to readers without a specialized background, Part I establishes foundations through cell analysis history and microfluidic fundamentals. Part II explores biochemical analysis advancements from single-cell proteomics to secretome profiling. Part III examines cellular biophysical properties including stiffness and migration, while Part IV covers manipulation techniques for cell movement and intracellular delivery. These chapters demonstrate how microfluidic technologies advance biology and translational medicine through adaptability and precision.
This comprehensive guide serves as a key reference for researchers, students, and professionals at the intersection of biology, engineering, and medicine.
Contents
Chapter 1 Cell Analysis Overview Chapter 2 Fundamentals of Microfluidics Chapter 3 Impedance based one-step cell characterization and preparation for mass spectrometry analysis Chapter 4 Advances in Microfluidic Technologies for Immunoassays and Biomarker Monitoring Chapter 5 Cellular Stiffness and Deformability: Mechanisms, Measurement, and Modern Insights Chapter 6 Cellular Stiffness and Deformability: From Nanoscale Mechanics to Microfluidic Morpholomics Chapter 7 Profiling Collective and Individual Cell Migration using Biomaterial and Microfluidic Technologies Chapter 8 Optoelectronic Tweezers Technology for Cell Manipulation and Analysis Chapter 9 Advances and Challenges in Membrane-Disruption-Based Microfluidic Intracellular Delivery
