Full Description
This comprehensive reference book on an essential mitochondrial enzyme, cytochrome c oxidase (COX), is the culmination of four decades of work. This enzyme's biochemical activity originated and evolved more than a billion years ago. Nevertheless, its contributions to the function of neurons remain relatively obscure. COX is not just a housekeeping enzyme engaged in energy generation, but a treasure trove for discovering numerous neuronal properties chronicled in this book. Various methods of studying COX in neurons are described, including biochemistry, histochemistry, cytochemistry, immunohistochemistry, in situ hybridization, and gene isolation. These methods revealed that COX also plays a critical role in rescuing the metabolic integrity of neurons exposed to toxins. The goal of this book is to raise the curiosity of readers about this invaluable enzyme so researchers will continue the quest to uncover neuronal properties unveiled by this enzyme.
Key Features
A comprehensive book on cytochrome c oxidase in neurons
Covers all major neuroscientific discoveries of cytochrome c oxidase in the mammalian nervous system
Provides a broad range of themes from systems, through cellular, to molecular
Contains not only the major discoveries but also various techniques for analyzing cytochrome c oxidase, plus a comprehensive list of references
Describes the mechanisms of energy regulation in neurons and how energy metabolism and neuronal activity are tightly coupled at the cellular and molecular levels
Documents the role of cytochrome c oxidase in uncovering a striking synaptic imbalance during the critical periods of both brain stem respiratory system and visual cortical development
Contents
Author Biography. Preface. Acknowledgments. Chapter 1 Introduction: Cytochrome c Oxidase. Chapter 2 A Sensitive Indicator of Metabolic Plasticity in Neurons. Chapter 3 Metabolic Diversity Among Cell Types and Segments of the Same Neuron: The Importance of Dendrites. Chapter 4 Uncovering a New Region in the Primate Visual Cortex: Puffs, Patches, Blobs, Dots, or Spots. Chapter 5 Metabolic Plasticity in the Adult Primate Visual Cortex: Not All Adult Neurons Respond Alike to the Same Functional Insult. Chapter 6 Role of Cytochrome c Oxidase in Photobiomodulation. Chapter 7 Molecular Mechanisms of Regulating the Bigenomic Cytochrome c Oxidase in Neurons. Chapter 8 Nuclear Respiratory Factor 1 (NRF-1): A Transcriptional Regulator of All 13 COX Subunit and Na+/K+-ATPase Genes in Neurons. Chapter 9 Nuclear Respiratory Factor 2 (NRF-2): A Transcriptional Regulator of All 13 COX Subunit Genes in Neurons. Chapter 10 Specificity Proteins 1 (Sp1) and 4 (Sp4): Transcriptional Regulators of All 13 COX Subunit and Na+/K+-ATPase Genes in Neurons. Chapter 11 Peroxisome Proliferator-Activated Receptor-γ Coactivator 1α (PGC-1α): A Transcriptional Coactivator in Regulating COX in Neurons. Chapter 12 Tight Coupling of Neuronal Activity and Energy Metabolism: NRF-1 Transcriptionally coregulates Mediators of Glutamatergic Neurotransmission, Energy Consumption, and Energy Generation. Chapter 13 NRF-2 Transcriptionally coregulates Genes for Glutamatergic Neurotransmitter Receptors, COX, and BDNF. Chapter 14 Sp4 Mediates Tight Coupling of Neuronal Activity and Energy Metabolism by Transcriptionally Coregulating Glutamatergic, GABAergic, Na+/K+-ATPase, and COX Genes in Neurons. Chapter 15 Critical Period of Postnatal Brain Stem Respiratory System Development in Rats. Chapter 16 Critical Period of Postnatal Primary Visual Cortical Development in Rats. Chapter 17 Role of Cytochrome c Oxidase in Human Diseases: Inherited Mitochondrial DNA Mutations, Nuclear DNA Mutations, and Neurodegenerative Diseases. Chapter 18 Methods for Analyzing COX: Histochemistry, Cytochemistry, Generation of Antibodies, Immunohistochemistry, Biochemistry, and Molecular Biological Techniques. Epilogue: Lessons Learned and Future Questions. References. Author Index. Subject Index.
