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基本説明
Offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering.
Full Description
Step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure.
Written by leading experts in the field, Culture of Cells for Tissue Engineering offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures.
Features of the text include:
Easy-to-use format with a two-part organization
Logically organized—part one discusses cell sourcing, preparation, and characterization and the second part examines specific engineered tissues
Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies
End-of-chapter features include useful listings of sources for reagents, materials, and supplies, with the contact details of the suppliers listed at the end of the book
A section of elegant color plates to back up the figures in the chapters
Culture of Cells for Tissue Engineering gives novice and seasoned researchers in tissue engineering an invaluable resource. In addition, the text is suitable for professionals in related research, particularly in those areas where cell and tissue culture is a new or emerging tool.
Contents
Preface vii
List of Abbreviations xi
Part I: Cell Culture
1. Basic Principles of Cell Culture 3
R. Ian Freshney
2. Mesenchymal Stem Cells for Tissue Engineering 23
Donald P. Lennon and Arnold I. Caplan
3. Human Embryonic Stem Cell Culture for Tissue Engineering 61
Shulamit Levenberg, Ali Khademhosseini, Mara Macdonald, Jason Fuller, and Robert Langer
4. Cell Sources for Cartilage Tissue Engineering 83
Brian Johnstone, Jung Yoo, and Matthew Stewart
5. Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering 113
Henning Madry
Part II: Tissue Engineering
6. Tissue Engineering: Basic Considerations 131
Gordana Vunjak-Novakovic
7. Tissue Engineering of Articular Cartilage 157
Koichi Masuda and Robert L. Sah
8. Ligament Tissue Engineering 191
Jingsong Chen, Jodie Moreau, Rebecca Horan, Adam Collette, Diah Bramano, Vladimir Volloch, John Richmond, Gordana Vunjak-Novakovic, David L. Kaplan, and Gregory H. Altman
9. Cellular Photoencapsulation in Hydrogels 213
Jennifer Elisseeff, Melanie Ruffner, Tae-Gyun Kim, and Christopher Williams
10. Tissue Engineering Human Skeletal Muscle for Clinical Applications 239
Janet Shansky, Paulette Ferland, Sharon McGuire, Courtney Powell, Michael DelTatto, Martin Nackman, James Hennessey, and Herman H. Vandenburgh
11. Engineered Heart Tissue 259
Thomas Eschenhagen and Wolfgang H. Zimmermann
12. Tissue-Engineered Blood Vessels 293
Rebecca Y. Klinger and Laura E. Niklason
13. Tissue Engineering of Bone 323
Sandra Hofmann, David Kaplan, Gordana Vunjak-Novakovic, and Lorenz Meinel
14. Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering 375
Peter I. Lelkes, Brian R. Unsworth, Samuel Saporta, Don F. Cameron, and Gianluca Gallo
15. Tissue Engineering of the Liver 417
Gregory H. Underhill, Jennifer Felix, Jared W. Allen, Valerie Liu Tsang, Salman R. Khetani, and Sangeeta N. Bhatia
Suppliers List 473
Glossary 483
Index 491
