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Full Description
The most complete, up-to-date reference on antigen retrieval and immunohistochemistry An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment.
Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects.
Featuring contributions from a worldwide group of leading experts and research scientists in the field, this important work:
Summarizes the key problems in the four fields of antigen retrieval
Discusses the advances of AR techniques and their applications
Provides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell/tissue sample preparation, and standardization and development of various techniques to meet the future needs of¿clinical and research molecular analysis
Encourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC
Includes an appendix of related laboratory protocols
Antigen Retrieval Immunohistochemistry Based Research and Diagnostics is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products.
Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems / Kai Chang, Series Editor
Contents
Preface ix
Contributors xv
Part I Recent Advances in Antigen Retrieval Techniques and Its Application 1
1 Standardization of Antigen Retrieval Techniques Based on the Test Battery Approach 3
Shan-Rong Shi and Clive R. Taylor
2 Extended Application of Antigen Retrieval Technique in Immunohistochemistry and in Situ
Hybridization 25
Shan-Rong Shi and Clive R. Taylor
3 Extraction of Dna/rna From Formalin-fixed, Paraffin-embedded Tissue Based on the Antigen Retrieval Principle 47
Shan-Rong Shi and Clive R. Taylor
Part II Standardization of Immunohistochemistry 73
4 Key Issues and Strategies of Standardization For Quantifiable Immunohistochemistry 75
Shan-Rong Shi, Kevin A. Roth, and Clive R. Taylor
5 Standardization of Immunohistochemistry Based on Antigen Retrieval Technique 87
Shan-Rong Shi and Clive R. Taylor
6 Standard Reference Material: Cell Line Development and Use of Reference Cell Lines as Standards for External Quality Assurance of Her2 Ihc and Ish Testing 101
Bharat Jasani, Vicky Reid, Colin Tristram, Jeremy Walker, Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim, and Keith Miller
7 Peptides as Immunohistochemistry Controls 123
Steven A. Bogen and Seshi R. Sompuram
8 Standard Reference Material: Protein- Embedding Technique and Design of Bar Code 141
Shan-Rong Shi, Jiang Gu, and Clive R. Taylor
9 the Pros and Cons of Automation for Immunohistochemistry From the Prospective Of the Pathology Laboratory 151
David G. Hicks and Loralee McMahon
10 Image Analysis in Immunohistochemistry 165
Alton D. Floyd
Part III Tissue/cell Sample Preparation 187
11 Tissue Cell Sample Preparation: Lessons From the Antigen Retrieval Technique 189
Shan-Rong Shi and Clive R. Taylor
12 Mechanisms of Action and Proper Use of Common Fixatives 195
Richard W. Dapson
13 Cell Sample Preparation for Clinical Cytopathology: Current Status and Future Development 219
Yan Shi and Patricia G. Wasserman
14 Design of a Tissue Surrogate to Examine Accuracy of Proteomic Analysis 235
Carol B. Fowler, Jeffrey T. Mason, and Timothy J. O'leary
Part IV Molecular Mechanism of Antigen Retrieval Technique 251
15 Study of Formalin Fixation and Heat-induced Antigen Retrieval 253
Jeffrey T. Mason, Carol B. Fowler, and Timothy J. O'leary
16 a Linear Epitopes Model of Antigen Retrieval 287
Steven A. Bogen and Seshi R. Sompuram
17 Ph Or Ionic Strength of Antigen Retrieval Solution: a Potential Role for Refolding During Heat Treatment 303
Shuji Yamashita
18 Commentary: Future Directions 323
Timothy J. O'Leary, Carol B. Fowler, David L. Evers, Robert E. Cunningham, and Jeffrey T. Mason
Part V Proteomic Analysis of Protein Extracted From Tissue/cells 333
19 Techniques of Protein Extraction From Ffpe Tissue/cells for Mass Spectrometry 335
Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason
20 Application of Shotgun Proteomics to Formalin-fixed and Paraffin-embedded Tissues 347
Brian M. Balgley
21 Visualizing Protein Maps in Tissue 369
Masahiro Mukai and Mitsutoshi Setou
22 Symbiosis of Immunohistochemistry and Proteomics: Marching to a New Era 391
Shan-Rong Shi, Brian M. Balgley, and Clive R. Taylor
Appendix Related Laboratory Protocols 399
Index 413
