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Full Description
Design and Applications of Cell-Free Systems, Part B, Volume 219 offers the latest information on cell free systems for the expression of transmembrane proteins, enzymes, proteins, therapeutics, vaccines, and uses in the biotransformation, translational modification, self-assembly of nano-biomaterials, and in synthetic biology design, challenges, and opportunities. It will help students, researchers, scientists, clinicians, stakeholders, policymakers, and practitioners understand cell-free systems and their applications in many areas. The book represents an important component in the field of biochemistry, duly acknowledging the pioneering effort made by Nobel laureate Eduard Buchner for discovery of fermentation in yeast cell extracts (YCE).
CFS requires core molecular machinery, including RNA polymerase, translational apparatus (ribosomes, tRNA synthases, and translation factors), energy-generating molecules, and their cofactors, substrates, and DNA or plasmid templates for obtaining desired products. CFS is a simple, rapid, and sensitive platform, which is devoid of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and machineries required for the synthesis of the desired proteins.
Contents
1. Cell-Free Systems for Expression of Transmembrane Protein
Mustafeez Mujtaba Babar
2. Cell free systems for expression of proteins and enzymes
Nisheeth Agarwal
3. Cell free systems for production of therapeutics
Gholamreza Abdi
4. Cell-free systems for vaccine production
Dinh-Toi Chu
5. Cell free systems for production of chemicals
Gholamreza Abdi
6. Cell free systems for biotransformation
Mustafeez Mujtaba Babar
7. Cell free systems for post-translational modifications
Vijai Singh
8. Cell free systems for assembly of nanobiomaterials
A. Vishnu Kirthi
9. Innovations in Cell-Free Systems via Microfluidic Approaches
Samar Damiati
10. Challenges and opportunities in cell-free systems
Dinh-Toi Chu
