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Full Description
Mucins are large, heavily glycosylated proteins that play critical roles in protecting and lubricating epithelial surfaces throughout the body, especially in the gastrointestinal, respiratory, and reproductive tracts. They are central to host-microbe interactions, immune modulation, and barrier function, and their complex structures and diverse functions make them both scientifically fascinating and technically challenging to study. Mucins serves as a valuable resource for researchers aiming to investigate mucin biology in greater depth, offering a wide range of experimental approaches and analytical strategies. It presents a comprehensive exploration of mucin biology and glycoproteomics, featuring cutting-edge methodologies and insights from leading researchers across the field. Topics span from enrichment and isolation techniques, mucinase characterization, and advanced glycoproteomic workflows to clinical applications such as lectin-based assays and mucin quantification. The chapters delve into innovative tools including CRISPR screens, cell-based mucin arrays, cryoEM, MD simulations, and microscopy, as well as synthetic approaches to mucin glycopeptides and selective sulfation. Special focus is given to mucin interactions with microbiota and pathogens, their roles in innate immunity and inflammatory bowel disease, and emerging technologies like FRET-based screening and glycan/lectin arrays. This multidisciplinary volume integrates biochemistry, microbiology, structural biology, and clinical science, serving as a foundational reference for researchers investigating mucins and their complex biological functions.
Contents
1. Enrichment/isolation, glycoproteomics
Stacy Malaker, Shane Finn and Sarah Lowery
2. Mucinase cleavage characterization and usage in glycoproteomics
Stacy Malaker, Alexandra Steigmeyer and Joann Chongsaritsinsuk
3. Fluorescent protein-based FRET substrates for activity screening of mucinases
Alisdair Boraston
4. Development of clinical lectin assays based on MS glycomic discovery
Niclas Karlsson, Ali Reza Afshari and Mary Solbego
5. Enzymatic selective sulfation of synthetic mucins
Jessica Kramer
6. cryoEM
Deborah Fass
7. Cell-based mucin arrays and glycoengineering
Yoshiki Narimatsu, Christian Bull, Henrik Clausen and Felix Goerdeler
8. CRISPR screens to characterize and identify glycan-binding proteins
Simon Wisnovsky
9. MD Simulations
Fiona Kearns
10. Interactions with pathogenic bacteria
Katharina Ribbeck
11. Interactions with microbiota
Eric Martens
12. Covalent crosslinking of mucins
Thomas Crouzier
13. Liquid Glycan and Liquid Lectin Array-based exploration of musin recognition
Ratmir Derda
14. Quantification of mucins
Sumaira Z. Hasnain
15. Mucins in IBD
Kirk Bergstrom
16. Investigatins mucins and innate immunity
Thaher Pelaseyed
17. Generating synthetic mucin glycopeptides
Peter Seeberger, Kabita Pradhan and Charlotte Völkel
18. Microscopy and imaging
Malin E.V. Johansson, George M H Birchenough, Elisabeth E L Nyström and Brendan Dolan
