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Full Description
Cyclodextrin Glucanotransferase: A Comprehensive Guide from Gene to Applications explores the role of cyclodextrin glucanotransferase (CGTase) in starch degradation and its applications. Sections cover the production of CGTase by different bacterial genera, including Bacillus spp., and purification strategies. It delves into the structural and functional characteristics of CGTase, highlighting its catalytic mechanisms, substrate specificity, and factors affecting enzyme activity and stability. Additional sections explore recombinant CGTases, their properties, and advancements in protein engineering and enzyme immobilization. With a strong focus on practical applications, the book discusses inclusion complex formation and a wide range of industrial uses in pharmaceuticals, textiles, bioremediation, and food.
Detailed case studies and real-world examples illustrate how CGTase and cyclodextrins can address specific challenges and drive innovation in these fields. This book is an essential resource for applied science researchers working in the field of starch processing enzymes, providing them with the knowledge to advance their research and development. It also serves as a valuable reference for researchers in the starch processing industries, offering insights into the latest advancements and practical applications of CGTase and cyclodextrins.
Contents
Section I: Introduction
1. Introduction to α-amylase Family
2. Cyclodextrin Glucanotransferase: An Overview
3. Structure of Cyclodextrin Glucanotransferases
Section II: Mechanism and Cyclodextrin Glucanotransferase Production
4. Catalytic mechanisms of Cyclodextrin Glucanotransferases
5. Cyclodextrin Glucanotransferase from Bacillus spp.
6. Cyclodextrin Glucanotransferase from other bacterial genera
Section III: Purification and Engineering of Cyclodextrin Glucanotransferases
7. Cloning and Overexpression of CGTases
8. Extraction, Purification, and Characterization of Cyclodextrin Glucanotransferases
9. Protein Engineering of Cyclodextrin Glucanotransferases
10. Immobilization of Cyclodextrin Glucanotransferases
Section IV: Cyclodextrins and their Applications
11. Structure and Properties of Cyclodextrins
12. Current Status of Cyclodextrin Production
13. Large Ring Cyclodextrins
14. Applications of Cyclodextrins
