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Full Description
Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique including isolation techniques for DNA and RNA, gene cloning, PCR, ELISA, and cell culture. These protocols include a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.
This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.
Contents
1. Isolation of Genomic DNA: From animal cells, tissues, plant cell, and bacteria
2. Isolation of RNA
3. PCR (Polymerase Chain reaction)
4. Real-time (Quantitative) PCR
5. Electrophoresis
6. 3' RACE
7. 5'RACE
8. Isolation of Plasmid
9. Competent cell preparation
10. Gene Cloning
11. End labelling of DNA
12. SDS and NATIVE PAGE
13. Western Blotting
14. EMSA (Electrophoretic Mobility Shift Assay)
15. Silver staining
16. Cell Culture
17. MTT assay
18. Lactate Dehydrogenase assay
19. Luciferace assay
20. Co-IP
21. Protein purification
22. Sub cellular fractionation
23. DNA foot printing
24. Chromatin Immunoprecipitation
25. Fluorescent activated cell sorting (FACS)
26. Basics of Bioinformatics
27. GST Pulldown assay
28. Fluorescence In situ Hybridization
29. Yeast Two Hybrid Assay
30. ELISA (Enzyme Linked Immuno Sorbent Assay)
