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Full Description
For general microbiology laboratory courses. Containing 57 thoroughly class-tested and easily customizable exercises, Laboratory Experiments in Microbiology, Eleventh Edition, provides engaging labs with instruction on performing basic microbiology techniques and applications for undergraduate students in diverse areas, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The perfect companion to Tortora/Funke/Case's Microbiology: An Introduction or any introductory microbiology text, the Eleventh Edition features fourteen new Part-opening Case Studiesthat introduce students to a real world scenario or health-oriented case that connects the lab exercises to an engaging, familiar context. Updates to the new ASM BSL-2 safety lab protocol enhance flexibility and customization options for the instructor. MasteringMicrobiology's newly updated prelab quizzes along with MicroLab Tutors and Lab Technique Videos ensure students arrive prepared for each lab and provide additional review opportunities.
Contents
Part One Microscopy 1. Use and Care of the Microscope2. Examination of Living MicroorganismsPart Two Handling Bacteria3. Microbes in the Environment4. Transfer of Bacteria: Aseptic TechniquePart Three Staining Methods5. Preparation of Smears and Simple Staining6. Negative Staining7. Gram Staining8. Acid-Fast Staining9. Structural Stains (Endospore, Capsule, and Flagella)10. Morphological UnknownPart Four Cultivation of Bacteria11. Isolation of Bacteria by Dilution Techniques12. Special Media for Isolating BacteriaPart Five Microbial Metabolism13. Carbohydrate Catabolism14. Fermentation15. Protein Catabolism, Part 116. Protein Catabolism, Part 217. Respiration18. Unknown Identification and Bergey's ManualPart Six Microbial Growth19. Oxygen and the Growth of Bacteria20. Determination of a Bacterial Growth Curve: The Role of Temperature21. BiofilmsPart Seven Control of Microbial Growth22. Physical Methods of Control: Heat23. Physical Methods of Control: Ultraviolet Radiation24. Chemical Methods of Control: Disinfectants and Antiseptics25. Chemical Methods of Control: Antimicrobial Drugs26. Effectiveness of Hand ScrubbingPart Eight Microbial Genetics27. Regulation of Gene Expression. 28. Isolation of Bacterial Mutants. 29. Transformation of Bacteria. 30. DNA Fingerprinting. 31. Genetic Engineering. 32. Ames Test for Detecting Possible Chemical Carcinogens. Part Nine The Microbial World 33. Fungi: Yeasts and Molds 34. Phototrophs: Algae and Cyanobacteria. 35. Protozoa. 36. Parasitic HelminthsPart Ten Viruses37. Isolation and Titration of Bacteriophages. 38. Plant Viruses. Part Eleven Interaction of Microbe and Host39. Epidemiology. 40. Koch's Postulate. Part Twelve Immunology41. Innate Immunity. 42. Agglutination Reactions: Slide Agglutination. 43. Agglutination Reactions: Microtiter Agglutination. 44. ELISA Technique. Part Thirteen Microrganisms and Disease 45. Bacteria of the Skin. 46. Bacteria of the Respiratory Tract. 47. Bacteria of the Mouth. 48. Bacteria of the Gastrointestinal Tract. 49. Bacteria of the Urogenital Tract. 50. Identification of an Unknown from a Clinical Sample. 51. Rapid Identification MethodsPart Fourteen Microbiology and the Environment52. Microbes in Water: Multiple-Tube Technique. 53. Microbes in Water: Membrane Filter Technique. 54. Microbes in Food: Contamination. 55. Microbes Used in the Production of Foods. 56. Microbes in Soil: The Nitrogen and Sulfur Cycles. 57. Microbes in Soil: Bioremediaton. APPENDICES. A. Pipetting. B. Dilution Techniques and Calculations. C. Use of the Spectrophotometer. D. Graphing. E. Use of the Dissecting Membrane. F. Use of the Membrane Filter. G. Electrophoresis. H. Keys to Bacteria. Index.
