Biological Small Angle Scattering : Theory and Practice (International Union of Crystallography Monographs on Crystallography)

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Biological Small Angle Scattering : Theory and Practice (International Union of Crystallography Monographs on Crystallography)

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  • 製本 Hardcover:ハードカバー版／ページ数 284 p.
  • 言語 ENG
  • 商品コード 9780199670871
  • DDC分類 548.80153543

Full Description

Small angle solution scattering (SAS) is increasingly being applied to biological problems. It is a complementary technique that, when applied in appropriate circumstances with carefully structured questions, can provide unique information not available from other techniques. While small angle solution scattering has been around for some time, a confluence of recent developments has dramatically enhanced its power. Intense third generation X-ray sources, low noise detectors, development of new algorithms and the computational power to take advantage of these have all matured, and use of free-electron x-ray laser sources is on the horizon. Whole new classes of experiments and analyses have been created as a result. These include the generation of molecular envelopes, the ability to do time-resolved studies, and the ability to account for structural changes using modelling based on the SAS data. The technical improvements have also reduced the amount of time and material needed to carry out an experiment. Beamtime at synchrotron sources is in demand, workshops on the subject are popular and researchers adopting the technique as part of their repertoire are growing. With these in mind, this book was written to guide structural biologists who may wish to adopt the technique, understand its strengths and weaknesses or just have a general interest in its potential.

Contents

PART I: INTRODUCTION
1: Introduction
PART II: THEORY OF SMALL ANGLE SCATTERING
2: Theoretical Background
3: Quantities directly measurable by Scattering
4: Three-Dimensional Modeling of SAXS Data
PART III: PRACTICAL ASPECTS OF SMALL ANGLE SCATTERING
5: Before the beamtime
6: During the beamtime
7: Analysis after the beamtime
8: Worked Examples of Data Collection, Processing, and Analysis
9: Dealing with imperfect samples
10: SAXS Instrumentation
11: High-throughput approaches to SAXS
12: Small Angle Neutron Scattering
PART IV: APPLICATIONS PAST, PRESENT, AND FUTURE
13: Examples of Biological Small Angle Scattering
14: Developments on the Horizon
15: Epilogue