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基本説明
Include isolation and fractionation of glycans and structural analysis of glycans by conventional methods as well as those sophisticated ones, such as NMR and mass spectrometry.
Full Description
An introduction to basic methods of analysing the structure of glycans attached to glycoproteins Comprehensive coverage - from simple methods to more sophisticated techniques The clear, step-by-step protocols are easy to follow and will enable the reader to determine the complete structure of glycans attached to proteins. With rapid development and heightened interest in carbohydrates of glycoproteins over recent years, this book provides an essential guide to this field for all levels of researchers. This book is intended for graduate students and post-doctoral researchers, in both an academic and industrial setting, who are interested in the analysis of carbohydrates and glycoproteins.
Contents
Isolation and characterization of glycoproteins; Compositional analysis of glycoproteins; Isolation and fractionation of N-linked oligosaccharides from glycoproteins; Release of N-linked oligosaccharides and fractionation by gel filtration; Fractionation of oligosaccharides by serial affinity chromatography with use of immobilized lectin columns; Fractionation of glycopeptides and oligosaccharides from glycoproteins by HPLC; Structural characterization of oligosaccharides from glycoproteins; Glycosidase treatment and other methods, including methylation analysis; FAM-MS and ES-MS of glycoproteins; Nuclear magnetic resonance spectroscopy of glycoprotein-derived carbohydrate chains; Structural characterization of N-glycans obtained from metabolically-radiolabelled glycoproteins; Structural characterization of mucin-type O-linked oligosaccharides; Characterization of nuclear and cytoplasmic glycoproteins; GPI membrane anchors.
