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Full Description
The Handbook of Biomedical Nonlinear Optical Microscopies provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections.
The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.
Contents
PART I. HISTORICAL DEVELOPMENT OF NONLINEAR OPTICAL SPECTROSCOPY AND MICROSCOPY; INTRODUCTION TO PART I: HISTORICAL DEVELOPMENT OF NONLINEAR OPTICAL MICROSCOPY AND SPECTROSCOPY; PART II. NONLINEAR OPTICAL SPECTROSCOPY; INTRODUCTION TO PART II: NONLINEAR OPTICAL SPECTROSCOPY; PART III. NONLINEAR OPTICAL INSTRUMENTS FOR MICROSCOPIC IMAGING AND ANALYSIS; INTRODUCTION TO PART III: NONLINEAR OPTICAL INSTRUMENTS FOR MICROSCOPIC IMAGING AND ANALYSIS: REVIEW AND FORECAST; PART IV. BIOMEDICAL APPLICATIONS OF NONLINEAR OPTICAL MICROSCOPY; INTRODUCTION TO PART IV: BIOMEDICAL APPLICATIONS OF NONLINEAR OPTICAL MICROSCOPY



