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Full Description
Biomolecular Interactions: Part A, Volume 166, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics in cell biology. Each chapter is written by an international board of authors.
Contents
1: Measuring the rapid kinetics of receptor-ligand interactions in live cells using NanoBRET
2: Evaluating functional ligand-GPCR interactions in cell-based assays
3: Assays for detecting arrestin interaction with GPCRs
4: BRET-based assay to specifically monitor β2AR/GRK2 interaction and β-arrestin2 conformational change upon βAR stimulation
5: Cannabinoid Receptor CB1 and CB2 Interacting Proteins: Techniques, Progress and Perspectives
6: Purinergic GPCR transmembrane residues involved in ligand recognition and dimerization
7: Nanobodies as Sensors of GPCR Activation and Signaling
8: Confocal and TIRF microscopy based approaches to visualize arrestin trafficking in living cells
9: Strategies for targeting cell surface proteins using multivalent conjugates and chemical biology
10: Identifying Plasmodium falciparum receptor activation using bioluminescence resonance energy transfer (BRET)-based biosensors in HEK293 cells
11: Methods for Binding Analysis of Small GTP-Binding Proteins with their Effectors
12: Protein-protein interactions in the brain: protocol for preparing rodent brain tissue for mass spectrometry-based quantitative- and phospho- proteomics analysis
13: Protein-Protein Interactions at a glance: protocols for the visualization of biomolecular interactions
14: Interactions between Noncoding RNAs as Epigenetic Regulatory Mechanisms in Cardiovascular Diseases
