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Full Description
Cell-Derived Matrices, Part A, Volume 156, provides a detailed description and step-by-step methods surrounding the use of three-dimensional cell-derived matrices for tissue engineering applications. Biochemical, biophysical and cell biological approaches are presented, along with sample results. Specific chapters cover Anisotropic cell-derived matrices with controlled 3D architecture, Generation of functional fluorescently-labelled cell-derived matrices by means of genetically-modified fibroblasts, Bi-layered cell-derived matrices, Engineering clinically-relevant cell-derived matrices using primary fibroblasts, Decellularized matrices for bioprinting applications, and much more.
Contents
Preface
David Caballero, Subhas C Kundu and Rui L Reis
Section 1: Decellularization and characterization methods
1.A step-by-step protocol for generating human fibroblast cell-derived completely biological extracellular matrix scaffolds
Dhavan Sharma, Morgan Ferguson and Feng Zhao
2.Preparation of osteogenic matrices from cultured cells
Carl A. Gregory, Eoin McNeill and Simin Pan
3.Decellularized hASCs-derived matrices as biomaterials for 3D in vitro approaches
Fátima Raquel Maia, Rui Luís Reis and Joaquim Miguel Oiveira
4.Characterization of the elastic properties of extracellular matrix models by atomic force microscopy
J. Otero, D. Navajas and J. Alcaraz
5.Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry
João C. Silva, Marta S. Carvalho, Ke Xia, Joaquim M. S. Cabral, Claúdia L. da Silva, Frederico C. Ferreira, Deepak Vashishth and Robert J. Linhardt
Section 2: Engineering cell-derived matrices with advanced characteristics
6.Engineering clinically-relevant human fibroblastic cell-derived extracellular matrices
Janusz Franco-Barraza, Kristopher S. Raghavan, Tiffany Luong and Edna Cukierman
7.Engineering cell-derived matrices with controlled 3D architectures for pathophysiological studies
Enrico Almici, David Caballero, Joan Montero Boronat and Josep Samitier Martí
8.Generation of fluorescent cell-derived-matrix to study 3D cell migration
Amélie Luise Godeau, Hélène Delanoë-Ayari and Daniel Riveline
9.Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM
Insung Yong, Seung Won Oh and Pilnam Kim
Section 3: Cell-derived matrices for biomedical applications
10.Cell-derived matrices (CDM)—Methods, challenges and applications
Cheng HW, Yuan MT, Li CW and Chan BP.
11.Boosting collagen deposition with a lysyl oxidase/bone morphogenetic protein-1 cocktail
Tamara Rosell-García and Fernando Rodríguez-Pascual
12.Generating specific cell-derived matrices from human trabecular meshwork cell cultures for mechanistic studies
Felix Yemanyi, Janice Vranka and VijayKrishna Raghunathan
13.Methods in cell biology: Cell-derived matrices
Elizabeth Pavez Loriè and Petra Boukamp
