Three-Dimensional Electron Microscopy (Methods in Cell Biology)

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Three-Dimensional Electron Microscopy (Methods in Cell Biology)

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  • 製本 Hardcover:ハードカバー版／ページ数 303 p.
  • 言語 ENG
  • 商品コード 9780128170182
  • DDC分類 570.2825

Full Description

Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics.

Contents

1. FIB-SEM of mouse nervous tissue: Fast and slow sample preparation
Anna M. Steyer, Andreas Schertel, Christos Nardis and Wiebke Möbius
2. Expedited large-volume 3-D SEM workflows for comparative microanatomical imaging
Gerald John Shami, Delfine Cheng and Filip Braet
3. Serial-section electron microscopy using automated tape-collecting ultramicrotome (ATUM)
Valentina Baena, Richard Lee Schalek, Jeff William Lichtman and Mark Terasaki
4. Serial block face-scanning electron microscopy for volume electron microscopy
Saskia Lippens, Anna Kremer, Peter Borghgraef and Chris Guérin
5. Combining serial block face and focused ion beam scanning electron microscopy for 3D studies of rare events
Christopher J. Guérin, Anna Kremer, Peter Borghgraef, Andy Y. Shih and Saskia Lippens
6. Yeast membraneless compartments revealed by correlative light microscopy and electron tomography
Guendalina Marini and Gaia Pigino
7. In situ analysis of male meiosis in C. elegans
Gunar Fabig, Anna Schwarz, Cynthia Striese, Michael Laue and Thomas Müller-Reichert
8. Software for automated acquisition of electron tomography tilt series
Guenter P. Resch
9. In situ cryo-electron tomography and subtomogram averaging of intraflagellar transport trains
Mareike Jordan and Gaia Pigino
10. CryoSTEM tomography in biology
Sharon G. Wolf and Michael Elbaum
11. Subtomogram averaging from cryo-electron tomograms
Kendra E. Leigh, Paula P. Navarro, Stefano Scaramuzza, Wenbo Chen, Yingyi Zhang, Daniel Castaño-Díez and Misha Kudryashev
12. Computational methods for stitching, alignment, and artifact correction of serial section data
Stephan Saalfeld
13. Content-aware image restoration for electron microscopy
Tim-Oliver Buchholz, Alexander Krull, Réza Shahidi, Gaia Pigino, Gáspár Jékely and Florian Jug